We compared three transport methods for the recovery of Pasteurella haemolytica from pharyngeal swabs collected under field conditions from 42 bighorn sheep (Ovis canadensis) in one captive and three free-ranging populations. Transport methods included: Amies medium with charcoal, transported on ice, and cultured on blood agar within 24 hr; direct plating on blood agar, transported on heating pads, and incubated at 37 C within 8 hr of collection; and phosphate buffered glycerol (PBG), transported on dry ice, and stored at − 70 C for 10 days before culture. Isolates of P. haemolytica were recovered from all 42 bighorn sheep with a mean (±SE) of 1.2 ± 0.1 (Amies), 1.3 ± 0.1 (blood agar), and 1.3 ± 0.1 (PBG) isolates per swab. No statistical differences (P > 0.05) were observed in the recovery of P. haemolytica isolates among the transport methods. However, based on our experience and results of this study, we recommend that if submission of samples to the laboratory is likely to be delayed, pharyngeal swabs be transported in PBG on dry ice and kept frozen until they are cultured. Viable samples can be maintained in PBG at − 70 C for several years.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 30 • No. 2