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1 July 2003 Use of Immunohistochemistry to Diagnose Chytridiomycosis in Dyeing Poison Dart Frogs (Dendrobates tinctorius)
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Abstract

Chytridiomycosis, caused by Batrachochytrium dendrobatidis, is an emerging disease of both wild and captive amphibians, posing a threat to their survival in many parts of the world. As the disease can be difficult to diagnose on routine pathologic sections, the purpose of this study was to develop an additional method for visualization. To accomplish this, immunohistochemical staining was applied to histologic skin sections from four experimentally infected Dyeing poison dart frogs (Dendrobates tinctorius). Staining of the positive tissue sections was distinct and readily visualized, making this technique a valuable ancillary diagnostic test for this important disease.

Van Ells, Stanton, Strieby, Daszak, Hyatt, and Brown: Use of Immunohistochemistry to Diagnose Chytridiomycosis in Dyeing Poison Dart Frogs (Dendrobates tinctorius)
Tracy Van Ells, James Stanton, Ann Strieby, Peter Daszak, Alex D. Hyatt, and Corrie Brown "Use of Immunohistochemistry to Diagnose Chytridiomycosis in Dyeing Poison Dart Frogs (Dendrobates tinctorius)," Journal of Wildlife Diseases 39(3), 742-745, (1 July 2003). https://doi.org/10.7589/0090-3558-39.3.742
Received: 15 September 2002; Published: 1 July 2003
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