Recent records of lichenicolous fungi from the Kharkiv region are provided. Twenty species were reported as new to the region. Pronectria gromakovae is described as a new species on Lecanora populicola. Didymocyrtis cladoniicola, D. epiphyscia, Epicladonia sandstedei, Katherinomyces cetrariae and Lichenoconium lichenicola are new to the forest-steppe zone of Ukraine. Physcia stellaris is reported as a new host species for Cladosporium licheniphilum. Notes on the currently known distribution of selected species in other Ukrainian regions are provided.