Teucrium chamaedrys is the most variable species in the genus Teucrium, fitting Kruckeberg's category of a bodenvag species. Six populations distributed on serpentine and 3 populations off serpentine were investigated, and 23 morphological features were studied by univariate and multivariate statistical analyses. The variation was higher for the vegetative features and not clearly expressed for the generative ones. In addition, metal concentration in the populations and soil were studied. The species tolerant to the serpentine conditions demonstrated specific morphological differences in stem length, stem length up to the first leave pair, and internode length between second and third leave pairs. Morphological differences, geographical isolation, and preliminary results on the karyology of T. chamaedrys in Bulgaria, suggest that the populations studied were different ecotypes.