Sixty percent of Maryland's odonate species are considered to be in need of conservation. To prioritize areas for the protection of biodiversity, the Maryland Department of Natural Resources (MD DNR) has identified 10 watersheds with the highest rates of occurrence of imperiled and rare stream species, including odonates. We examined the lotic-breeding odonate fauna of two of these high priority watersheds to determine the distribution and status of several imperiled odonate species in Maryland. Odonate nymphs from two Lower Potomac River basin watersheds, Zekiah Swamp Run and Breton Bay, were collected by volunteers and MD DNR's Maryland Biological Stream Survey from 2000–2010 and were identified to species level when possible. Thirty-four species were collected during this survey, 10 of which are state-listed species. The data collected in this survey detail the distributions, habitats, and microhabitats of rare odonates in two priority watersheds in Maryland that can be used to aid in the conservation of these species and their habitats.