We studied a remote and robust population of Actinemys pallida (Southwestern Pond Turtle) in the upper Río Santo Domingo (RSD) watershed, Sierra San Pedro Mártir, Baja California, Mexico. We conducted cursory assessments in 2014 and 2015 and intensive trap-based surveys in 2016 and 2022. We captured and marked 486 unique turtles a total of 597 times. Using closed-population models within years, we estimated the total abundance of one 3.8-km study reach to be 511.1 (CI = 395.7–686.5) turtles in 2016 and 663.8 (467.2–1011.0) turtles in 2022, equivalent to instream densities of 134.5 turtles/river-kilometer (rkm) in 2016 and 174.7 turtles/rkm in 2022. The proportion of juveniles among detected turtles was 0% in 2014, 4.6% in 2015, 21.7% in 2016 and 19.6% in 2022. In 73.5 trap-nights (TN), we recorded 532 captures, equivalent to an average of 7.24 turtles/TN or 0.30 turtles/trap-hour. The RSD population appears to be one of the largest known in Baja California and is relatively large across the range of the Southwestern Pond Turtle. Thirty-three turtles recaptured more than 1 year apart had moved up to 1.6 km downstream and 2.3 km upstream, averaging a net upstream movement of 240 m. Adult males and females both had an average straight carapace length (SCL) of 112 mm, which is small for Southwestern Pond Turtles, but expected in the regional context. In addition to a regionally significant population of Southwestern Pond Turtles, this isolated, perennial watercourse supports a micro-endemic fish and 2 regionally rare amphibians and warrants protection as a globally significant biodiversity reserve.