Fisher (Martes pennanti) occur broadly across northcentral North America, and are a species of conservation concern in most of western North America. Fisher reach the northwestern edge of their distributional range in the Yukon, Canada; however, their abundance and distribution has been poorly documented there. We used museum collections, trapping records, and sightings to update the relative abundance and distribution of Fisher in the Yukon between 1956 and 2009, with a particular emphasis on the last 3 decades. We compiled 100 records of Fisher that were trapped, observed, or whose tracks were noted in the Yukon. Harvest data suggested that Fisher may be increasing in relative abundance in the Yukon, but densities likely are very low. Anecdotal observations and harvest of Fisher in southwestern Yukon have increased in the last 10 to 20 y and suggest that they may be expanding their range westward. Trapping records and museum collections in adjacent Alaska and northwestern British Columbia corroborate records from the Yukon. Monitoring Fisher will be necessary to confirm changes in the abundance and distribution of Fisher in the Yukon and neighboring jurisdictions.