Fluorescence analysis of modern pollen grains is a well-established technique, but its use on fossil material is significantly less prevalent. A study focused on pollen and spore fluorescence and its indication was carried out using samples collected from a 14-m-deep exposed section at an abandoned fluvial terrace in the Khorat Plateau, the center of the Indochina peninsula. Fluorescent signals were well preserved in the deposits at various depths. Thirty-seven fluorescent pollen and spore taxa were discovered in six layers dated from Middle to Late Pleistocene. An alternative algorithm method was used to calculate the fluorescence intensity (FI) in the layers. The FI of each fossil pollen taxon, represented by an assigned value, is different among the layers throughout the section. Differences in the FI indicate varied preservation conditions. General linear analysis indicates that the total FI value is related to the pollen taxa but not to the deposit depth.