Pollen micromorphology and ultrastructure of Colchicum kotschyi and Colchicum robustum were examined by Light Microscopy (LM), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM). The observations showed that all quantitative characters of pollen micromorphology varied in the studied taxa. Although, pollen grains in both species were oblate. Two pollen types, two major exine ornamentations in the equatorial surface of pollen grain with two kinds of pore intine ornamentation were seen during this research. Regarding sporoderm data, the thickness of the pollen wall layer, state of the endexine layer, and number of intine layers of C. kotschyi were different from C. robustum. Sporoderm characters strongly supported the unique position of C. robustum resulting from previous palynological research.