Direct Optical Detection of Singlet Oxygen from a Single Cell¶

Ingo Zebger; John W. Snyder; Lars Klembt Andersen; Lars Poulsen; Zhan Gao; John D. C. Lambert; Uffe Kristiansen; Peter R. Ogilby

doi:10.1562/RC-065R.1

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Ingo Zebger,¹ John W. Snyder,¹ Lars Klembt Andersen,¹ Lars Poulsen,¹ Zhan Gao,¹ John D. C. Lambert,² Uffe Kristiansen,³ Peter R. Ogilby^1,*

¹aDepartment of Chemistry, University of Aarhus, Århus, Denmark
²bDepartment of Physiology, University of Aarhus, Århus, Denmark
³cDepartment of Pharmacology, The Danish University of Pharmaceutical Sciences, Copenhagen, Denmark

^**To whom correspondence should be addressed: Department of Chemistry, University of Aarhus, DK-8000 Århus, Denmark. Fax: (45)8619-6199 progilby@chem.au.dk

Abstract

Singlet oxygen has been detected in single nerve cells by its weak 1270 nm phosphorescence (a¹Δ_g→X³Σ_g⁻) upon irradiation of a photosensitizer incorporated in the cell. Thus, one can now consider the application of direct optical imaging techniques to mechanistic studies of singlet oxygen at the single-cell level.

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Ingo Zebger, John W. Snyder, Lars Klembt Andersen, Lars Poulsen, Zhan Gao, John D. C. Lambert, Uffe Kristiansen, and Peter R. Ogilby "Direct Optical Detection of Singlet Oxygen from a Single Cell," Photochemistry and Photobiology 79(4), 319-322, (1 April 2004). https://doi.org/10.1562/RC-065R.1

Received: 27 January 2004; Accepted: 1 February 2004; Published: 1 April 2004

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