Soil nitrogen mineralization strongly affects N availability, thus impacting the primary productivity in ecosystems. The seasonal environmental changes affect soil mineralization in restored sandy grasslands such as a studied mobile dune (MD), a semi-fixed dune (SFD), a fixed dune (FD) and a grassland (G). During the growing season, we examined the association of soil N mineralization rate with vegetation characteristics, soil properties and climatic factors through the multivariate stepwise regression model. The vegetation cover, species diversity, above- and belowground biomass, soil carbon, nitrogen, soil water content (SWC), pH, electrical conductivity, very fine sand, clay and silt fractions increased during sandy grassland restoration. The NH₄⁺-N concentration in MD and SFD was higher than that in FD and G, while NO₃^--N and inorganic N concentration showed a reverse trend. The NH₄⁺N, NO₃^--N and inorganic N concentrations in MD, SFD and FD reached to the highest values in June, while in G they were highest in May. The net mineralization and nitrification rates increased with sandy grassland restoration; both of these rates were much greater in June than in other months at all sites. Regression analysis showed that the NO₃^--N concentration, SWC, pH of the soil and precipitation could explain 75% of the total variation in net nitrification rate, and the NO₃^--N concentration and precipitation could explain 59% of the total variation in the net mineralization rate. These results illustrate that the sandy grassland restoration can enhance the soil N availability, with soil N mineralization mainly determined by the changes of the NO₃^--N concentration and precipitation.