Neuropterans are beneficial insects being generalist predators during both larval and adult stages. In Mexico, 349 neuropteran species have been recorded, but only two species are known from the state of Tlaxcala. We conducted a faunistic study and analysis of diversity of Coniopterygidae and Hemerobiidae at two sites within the municipality of Nanacamilpa, Tlaxcala. We collected monthly samples for a year using Malaise traps, with some sporadic sampling with nets and light traps. We identified a total of 19 species (8 Coniopterygidae and 11 Hemerobiidae) of which 11 are new records for the state and five are new records for the country. One of the brown lacewing species collected was only recently described from the study area in the Transmexican Volcanic Belt. Coniopterygids and hemerobiids showed three abundance peaks through the one-year sampling period at Los Búfalos (LB) study site, while a less defined pattern of two apparent peaks was observed at the Piedra Canteada (PC) site. Results of true alpha diversity (order 0) based on the 14 observed species model an expected six more species in LB and one more in PC, while species accumulations curves predict three to four more species at both LB and PC with an additional year of sampling. The amount of similarity between the two localities was 0.69 as they share 12 of the 19 species recorded.