Reactive oxygen species (ROS) generate a type of DNA damage called tandem lesions, two adjacent nucleotides both modified. A subcategory of tandem lesions consists of adjacent nucleotides linked by a covalent bond. Covalently linked tandem lesions generate highly characteristic liquid chromotography-tandem mass spectrometry (LC-MS/MS) elution profiles. We have used this property to comprehensively survey X-irradiated DNA for covalently linked tandem lesions. A total of 15 tandem lesions were detected in DNA irradiated in deoxygenated aqueous solution, five tandem lesions were detected in DNA that was irradiated in oxygenated solution.
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29 October 2012
Covalently Linked Tandem Lesions in DNA
Helen B. Patrzyc,
Jean B. Dawidzik,
Edwin E. Budzinski,
Harold G. Freund,
John H. Wilton,
Harold C. Box
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Radiation Research
Vol. 178 • No. 6
December 2012
Vol. 178 • No. 6
December 2012