Frequent, severe defoliation reduces grass production and can alter plant species composition in grasslands. Multipaddock rotational grazing has been proposed as a grazing strategy that may reduce the frequency and intensity of defoliation on palatable grass plants without altering stocking rates. Previous studies evaluated this hypothesis using small, homogeneous paddocks and nonadaptive rotation schedules and found small and inconsistent differences between continuous and rotational grazing systems. Using a stakeholder-driven collaborative adaptive management (CAM) framework, we conducted the first ranch-scale experimental investigation into tiller defoliation patterns in the context of adaptive multipaddock rotational grazing. We monitored tiller defoliation frequency and intensity in 10 paired 130-ha pastures assigned to either a collaborative adaptive multipaddock rotational grazing treatment (CARM, one livestock herd) or a season-long continuous grazing treatment (traditional rangeland management [TRM]; 10 separate herds) in shortgrass steppe. Consistent with previous studies, we observed that frequencies of grazing and regrazing on a palatable, cool-season grass (western wheatgrass, Pascopyrum smithii) were much more sensitive to stocking rate than grazing system. Under moderate stocking rates used in both CARM and TRM treatments, roughly two-thirds of western wheatgrass tillers remained ungrazed annually, regardless of grazing system. Thus, season-long rest is present in season-long continuous and rotational grazing systems. Frequencies of tiller regrazing were low (5–15%) and similar between CARM and TRM treatments. Although defoliation patterns were similar between treatments at the whole-ranch scale, CARM enhanced spatial and temporal heterogeneity in defoliation frequencies among individual pastures. Pastures grazed earlier in the season or for longer experienced more defoliation. Managers implementing adaptive, multipaddock rotational grazing could use this heightened and predictable variability to strategically manage impacts of grazing on western wheatgrass at the individual pasture scale. The CAM model enabled our team to identify and directly address key stakeholder hypotheses and resulted in coproduction of management-relevant research.