Arbuscular mycorrhizal fungi (AMF) are drivers of plant communities as they influence plant competition by increasing plants' root absorptive surface. As such, AMF could be an important tool for ecological restoration. However, a lack of research using AMF in sagebrush steppe communities implies a need for more assessments of its efficacy. Here, we assessed the ability of a commercial inoculum to colonize and influence growth of three native Artemisia spp. (sagebrush) shrubs and the exotic Taeniatherum caput-medusae (medusahead). We expected to increase colonization and biomass production with the addition of the inoculum. Before testing the inoculum, its viability was examined using Trifolium incarnatum (crimson clover) in autoclaved soil. Next, a factorial greenhouse experiment was conducted to evaluate the target species when grown in live or autoclaved field soil from a disturbed (early seral) site. Inoculation resulted in an average root colonization of 24.82% ± 4.86% for T. incarnatum but failed to colonize any of the sagebrush steppe species in autoclaved soil and had little to no influence on colonization in live soil. All sagebrush steppe species were colonized by AMF in live soil, and biomass production averaged 889 mg, 530 mg, 323 mg, and 1 852 mg, respectively; however, average biomass for all species was greater when grown in autoclaved soils (1 620 mg, 706 mg, 371 mg, and 3 902 mg, respectively). Our results reveal differential effects of a commercial AMF inoculum on root colonization of Artemisia spp. and T. incarnatum and highlight the need to assess the efficacy of AMF inoculum before field application.