A new species of genus Leistus Frölich, 1799, belonging to subgenus Evanoleistus Jedlička, 1965 is described and illustrated: L. rezabkovae sp. n. from Gansu (type locality: Lenglong Ling Mts., Wutai Ridge [pass], 70 km N Honggu, 3530 m, 36°58′16.6″N/102°48′03.6″E). It is compared with a similar species of the subgenus Evanoleistus, known from a mountain massif between the provinces Qinghai and Gansu. A check-list of all members of genus Leistus from the provinces Qinghai and Gansu is provided, comprising data on type locality and deposition of holotype.