Bats in the eastern US face many significant threats, and more information on the distribution, natural history, and condition of multiple bat species is needed for conservation planning and management. To address this need for the northwestern mountain region of North Carolina, we conducted a large-scale, 3-day bat blitz in 2011 at 30 mist-net sites across 6 counties. We documented the presence of 8 species and obtained new county and summer records for 7 of those 8. Most notably, in McDowell County we documented Lasiurus seminolus (Seminole Bat), a species associated with Coastal Plain habitats and for which there were no previous records in the northwestern mountain region of North Carolina. “Bio-blitz” inventories have many benefits beyond scientific results, including opportunities to share research ideas, foster new collaborations, train students in techniques, and provide educational programs.