Amsonia ludoviciana (Louisiana Bluestar), in the Apocynaceae family, is a rare herbaceous perennial plant species found in the ecotones of granite rock outcrops of Georgia as well as the pine flatwoods in Louisiana, Mississippi, and Texas. This research focused on using fluorescently tagged primers to amplify polymorphic microsatellite loci as a way to characterize the population genetic structure of the rare Louisiana Bluestar in Georgia. We sampled 6 populations across the Georgia range of Louisiana Bluestar. We employed 8 microsatellite loci to detect variation within and among Louisiana Bluestar populations and to detect patterns in population structure and dispersal. Values of the genetic-diversity estimator G_st suggested that the majority of the sampled populations demonstrated moderate genetic similarity. Further, Geneland software indicated the presence of 3 clusters. From our population genetic analyses, it can also be inferred that there is substantial gene flow between populations of Louisiana Bluestar in the state of Georgia.