Discarded bottles were inspected for skeletal remains at 220 roadside sites along the southeastern Blue Ridge escarpment of North Carolina, South Carolina, and Georgia as a technique to examine the regional distributions of shrews. Vertebrate remains were found at approximately 63% of our study sites and in 4.5% of the open bottles we examined. Bottles collected a total of 553 specimens of small mammals representing 5 species of shrews and 6 species of rodents. The Northern Short-tailed Shrew (Blarina brevicauda) and the Smoky Shrew (Sorex fumeus) were abundant and distributed throughout the region, although Smoky Shrews were more strongly associated with mesic environments and higher altitudes ( = 940.1 m ± 25.4 m). The Masked Shrew (S. cinereus) and the Southeastern Shrew (S. longirostris) exhibited contiguous allopatry, with Masked Shrews occurring exclusively in mesic forest habitats at high elevations ( = 1126.7 ± 27.4 m), and Southeastern Shrews occurring only in xeric habitats at lower elevations ( = 503.7 ± 64.9 m). Our study demonstrates the utility of discarded bottles as a quick and effective alternative method for surveying shrews, without the added mortality that occurs from pitfall- or snap-trapping.