With the aim to provide bases to define a regional management strategy of insecticides, the susceptibility of populations of Aedes aegypti from three regions of Guerrero, Mexico, to the insecticides malathion, temefos, chlorpyrifos, pirimiphosmetil, permethrin, lambda-cyhalothrin, propoxur, and spinosin was determinated, using the New Orleans population as a reference. Commercial and technical-grade insecticides were evaluated by residual application in larvae of early fourth instar according to the methodology proposed by the World Health Organization. The mortality was recorded 24 hours after application. Probit analysis was used to determinate the response lines log dose-mortality, LC₅₀ values and the resistant factor for each product. The results indicate that the three populations of A. aegypti were resistant to the insecticide lambda cyhalothrin, and the populations from Acapulco and Costa Chica were moderately resistant to pirimiphos methyl. In contrast, the three field populations were susceptible to malathion, temephos, chlorpyrifos, permethrin, propoxur, and spinosin. However, although some products were found to be effective, it is recommended to use them in rotation combining different modes of action and in conjunction with other alternatives, as removal of breeding sites, to preserve the life of the authorized insecticides. This study provides useful reference information for the development and implementation of strategies for managing and monitoring local insecticide resistance of A. aegypti in Guerrero, Mexico.