The aim of this study was to assess the biodiversity of mosquito species in houses of the five ecological regions and update the checklist of species previously reported in the state of Tabasco. In total, 325 dwellings at 43 localities in the five ecological regions were sampled. Collection was by entomological CDC backpack aspirator. The mosquitoes captured were identified using published taxonomic keys and the aid of a stereomicroscope. In total, 4,622 mosquitoes of 15 species in 10 genera were captured. Two species not previously reported in Tabasco State were found: Aedes (Ochlerotatus) angustivittatus (Dyar and Knab, 1907) and Mansonia dyari Belkin, Heinemann & Page, 1970. The mosquito fauna of the state of Tabasco, including the new species are 55 species in 17 genera. Most species found transmit diseases to humans and should be included in government mosquito control programs.