Introduction and spread of Euwallacea species in Mexico are a significant threat to production of avocado (Persea americana Mill.) and other potential hosts in urban and natural areas. Biological control with entomopathogenic fungi suggests an alternative to maintain pest abundance below the economic threshold. Nevertheless, isolation and characterization of strains capable of infecting Euwallacea spp. in local environmental conditions were previously required. Derived from the collection of natural enemies of Kuroshio shot hole borer, Euwallacea sp. nr. fornicatus (Eichhoff), mycosed adults were found in Populus sp. at Tijuana, Baja California, Mexico. Morphological (conidia and phialides were measured) and molecular (translation elongation factor and Bloc gene regions were sequenced) analyses resulted in the identification of two isolates of Beauveria bassiana (Bals.-Criv.) Vuill. Both strains produced greater growth between 25 and 27°C, and pathogenicity tests in Kuroshio shot hole borer ranged from 58.3 to 83.3%. It is the first report of B. bassiana in Euwallace sp. nr. fornicatus in Mexico.