Lasioseius is a genus widely distributed across the continents, found in many types of hosts. Two new species of blattisociid mites from Brazil, Lasioseius jorgeamadoi sp. nov. and Lasioseius gabrielae sp. nov., are described based on the morphology of adult females collected on buds of cocoa plants, Theobroma cacao L. (Malvaceae) and inflorescences of ornamental plants (Heliconiaceae and Musaceae) in the State of Bahia, and collected on sugar cane, Saccharum officinarum L. in the State of São Paulo. A key for the separation of females of the 14 species of Lasioseius recorded in Brazil is provided.