This issue commemorates the anniversary of Dr. Gary R. Bauchan's passing, on 12 January 2021. Dr. Bauchan, who was one of the victims of the Covid-19 pandemic, will be remembered for the many stunning images that he produced of mites. Mites have suffered from a lack of appreciation, perhaps partly because their small size makes it difficult to see them in detail. His arduous work at the Beltsville Agriculture Research Center has helped to transform the popular representation of mites from obscure creatures to interesting and dynamic organisms that are engaged in complex interactions with their environment. He mainly achieved this by helping to pioneer the use of cryoscanning electron microscopy on mites (Fig. 1). This is a technique that allows living mites to be frozen in place in order to study their morphology and behavior.
FIGURE 1.
SEM images of mites that were taken by Gary Bauchan. A. Dermatophagoides farina Bogdanov. B. Phyllocoptes fructiphilus Keifer. C. Trachymolgus purpureus Fisher & Dowling.
For those who experienced his sense of humor and boundless love for microscopy, Gary's departure has left an unfathomable void. Being in his presence was like riding in a magical roller coaster through clouds of liquid nitrogen mist into a wondrous microcosm. In Dr. Bauchan's Microscopy Unit, every day was an adventure to discover the unknown. He was a dedicated scientist of the Agricultural Research Service (ARS), who worked at Beltsville for 38 years. During that time, he authored and co-authored 253 publications, including 182 peer-reviewed papers in addition to 71 symposium articles, popular press articles, training videos, book chapters and conference abstracts.
Dr. Bauchan, Gary to all he worked with, was born in the scenic natural beauty of East Grand Rapids and raised in Wyoming, Michigan. He received his B.S. Biological Sciences degree in 1976 at Aquinas College, Michigan. In 1978, he obtained his M.S. Plant Sciences from St. Cloud State University, Minnesota and in 1996, his Ph.D. in Plant Cytogenetics and Breeding from his beloved Michigan State University, Michigan.
Gary was recognized as a worldwide expert in alfalfa genetics during his first 25 years at ARS. He was elected the President of the North American Alfalfa Improvement Conference in 1992 and served on the conference executive committee from 1988 until 2008. Dr. Bauchan received an Honorary Membership from the North American Alfalfa Conference in 2010 for “Outstanding contributions to the advancement of alfalfa improvement.” His research in plant genetics led him into the world of microscopy, and eventually low temperature scanning electron microscopy, to the study of microorganisms.
In 2007, he became the Director of the Electron & Confocal Microscopy Unit (ECMU) in the Soybean Genomics and Improvement Laboratory. In 2012, Dr. Bauchan coordinated the move of the ECMU into a newly renovated, $1.5 million space containing more than $3.5 million of scientific equipment, including state-of-the-art electron transmission and scanning electron microscopes, a confocal laser scanning microscope, wide field fluorescence microscope, and a high-resolution digital video light microscope. Under Dr. Bauchan's leadership, research in the ECMU resulted in 146 scientific articles plus 60 other publications in the past 12 years. During this period, his contributions were key in advancing research impacting a wide range of mites.
Dr. Bauchan and his team worked hand in hand with many different acarologists from around the world, obtained many high quality digital images of mites, which are freely accessible worldwide as identification and educational aids. Through LT-SEM and confocal microscopy, Dr. Bauchan provided new information that has added significantly to the understanding of morphology, behavior, biology, ecology, and the integration of molecular studies with CRYO-SEM morphological data. The websites http://idtools.org/id/mites/flatmites/and http://idtools.org/id/mites/beemites/ contain many scanning electron micrographs of flat mites and bee mites that are economically important from all around the world. Since it was launched in March 2012, the flat mite web page has had more than 130,000 visitors from 180 different countries and has proved to be an invaluable tool for researchers and regulatory officials from USDA-APHIS who want to identify mites.
Over the past 12 years, Dr. Bauchan participated in more than 200 different research projects at the Beltsville Agricultural Research Center (BARC), interacting with nearly every research unit on campus. He worked with scientists at USDA-APHIS, the National Park Service, Detroit Michigan's Belle Isle Aquarium, the American Museum in New York, the Field Museum in Chicago, States of California, Florida, Maryland, and Oregon Departments of Agriculture, Maryland's Department of Natural Resources, the National Academy of Sciences, the U.S. Geological Survey, and the Smithsonian Institute. He collaborated with scientists at 18 different U.S. universities and from 40 countries. He mentored 20 visiting scientists, 30 post-doctoral scientists, 16 graduate students and 3 undergraduate students. Gary loved teaching, and with his wife Francine, welcomed many of those he collaborated with and mentored into his home throughout the years for dinners and holidays.
Gary had an enormous amount of energy and was driven by insatiable curiosity. His photographs of microscopic organisms were stunning, showing us worlds that seemed too fantastic to be real. He revealed marvels that were previously unknown. His days started with a warm call to many of us telling the Cryo-SEM was running. There was no work he turned down, and there was no collaborator he ever turned away. He had a true passion for science, and he loved to talk about it over a strong cup of good coffee. We will always remember him.
Acknowledgements
Special thanks to the Bauchan Family for providing personal history. Reviews and comments were kindly supplied by the following: Dr. Howard Q. Zhang, BARC Center Director, USDA, ARS; Andrew Ulsamer, USDA, ARS, SEL; Dr. John Hammond, USDA, Arboretum, and Dr. Jenny J. Beard, Queensland Museum, Australia. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA; USDA is an equal opportunity provider and employer.
Publications
Bauchan, G.R. (1978) A cytogenetic study of the spider plant, Chlorophytum comosum (Liliaceae). M.A. Thesis, St. Cloud State University, 21 pp.
Bauchan, G.R. (1982) Cytogenetic studies of the dry beans, species of the genera Phaesolus and Vigna (Leguminosae). Dissertation Thesis. Michigan State University, 52 pp.
Terry-Lewandowski, V., Bauchan, G.R. & Stimart, D.P. (1984) Cytology and breeding behavior of interspecific hybrids and induced amphiploids of Zinnia elegans and Z. angustifolia. Canadian Journal of Genetics and Cytology, 26, 40–45. https://doi.org/10.1139/g84-007
Bauchan, G.R. & Elgin, J.H. Jr. (1984) A new chromosome number for the genus Medicago. Crop Science, 24, 193–195. https://doi.org/10.2135/cropsci1984.0011183X002400010046x
Small, E. & Bauchan, G.R. (1984) Chromosome numbers of Medicago sativa complex in Turkey. Canadian Journal of Botany, 62, 749–752. https://doi.org/10.1139/b84-110
Bauchan, G.R. (1985) Alfalfa: A model plant for biotechnology. Proceedings from the Sixth Eastern Forage Improvement Conference. Ithaca, NY., pp. 14–19.
Hammerschlag, F.A., Bauchan, G.R. & Scorza, R. (1985) Regeneration of peach plants from callus derived from immature embryos. Theoretical and Applied Genetics, 70, 248–255. https://doi.org/10.1007/BF00304907
Bauchan, G.R., Linkous, L-C.W. & Tai, W. (1987) Cytomixis in Agropyron cristatum. Genome, 28, 900–905. https://doi.org/10.1139/g87-129
Bauchan, G.R. (1987) Embryo culture of Medicago scutellata and M. sativa. Plant Cell, Tissue, and Organ Culture, 10, 21–29. https://doi.org/10.1007/BF00037493
Boyle, T.H., Stimart, D.P. & Bauchan, G.R. (1987) Influence of Zinnia angustifolia HBK genotype on embryonic and vegetative development of Z. angustifolia X Z. elegans Jacq. interspecific hybrids. Theoretical and Applied Genetics, 73, 716–723. https://doi.org/10.1007/BF00260782
Hammerschlag, F.A., Bauchan, G.R. & Scorza, R. (1987) Factors influencing in vitro multiplication and rooting of peach cultivars. Plant Cell and Organ Culture, 8, 235–242. https://doi.org/10.1007/BF00040950
Bauchan, G.R. (1988) North American Alfalfa Improvement Conference. Diversity, 16, 27–28.
Quiros, C.F. & Bauchan, G.R. (1988) The genus Medicago and the origin of the Medicago sativa complex. In: Hanson, A.A., Barnes, D.K. & Hill, R.R. (eds.) Alfalfa and Alfalfa Improvement. American Society of Agronomy, Agronomy Monograph, Madison, WI. 29., pp. 93–124. https://doi.org/10.2134/agronmonogr29.c3
Bauchan, G.R. (1990) USDA/ARS alfalfa research at the Beltsville Agricultural Research Center. WL Research Haymaker, pp. 5–6.
Campbell, T.A. & Bauchan, G.R. (1990) Stability of self-incompatibility and cross-compatibility in partially self-incompatible alfalfa clones. Canadian Journal of Plant Science, 70, 739–746. https://doi.org/10.4141/cjps90-091
Schaff, D.A., Koehler, S.M., Matthews, B.F. & Bauchan, G.R. (1990) In-situ hybridization of β-tubulin to alfalfa chromosomes. Journal of Heredity, 81, 480–483. https://doi.org/10.1093/oxfordjournals.jhered.a111029
Bauchan, G.R., Campbell, T.A., O'Neill, N.R. & Elgin, J.H. Jr. (1991) Self-incompatibility in two alfalfa populations. Crop Science, 30, 1205–1210. https://doi.org/10.2135/cropsci1990.0011183X003000060009x Bauchan, G.R., Diwan, N. & McIntosh, M.S. (1993) Development and evaluation of a core germplasm collection of annual Medicago species. Proceedings of the XVII International Grassland Congress. Palmerston, New Zealand, pp. 265–266. https://doi.org/10.2135/cropsci1990.0011183X003000060009x
Naess, S.K., Swartz, H.J. & Bauchan, G.R. (1993) Chromosome loss following interspecific hybridization in Rubus chamaemorus L. Acta Horticulture, 352, 421–428. https://doi.org/10.17660/ActaHortic.1993.352.61
Small, E., Bauchan, G.R., Salter, R., Brooks, B. & Auricht, G.C. (1993) A systematic comparison of morphological and seed proteins of early- and late-flowering forms of Medicago scutellata. Canadian Journal of Botany, 71, 183–192. https://doi.org/10.1139/b93-021
Bauchan, G.R., Diwan, N. & McIntosh, M.S. (1994) What are annual Medics? Proceedings of the North American Alfalfa Improvement Conference. Guelph, Ontario, Canada, p. 6.
Bauchan, G.R. (1994) Biotechnology: Facts and Promises. Twenty-Fourth National Alfalfa Symposium, pp. 191–194.
Bauchan, G.R. & Campbell, T.A. (1994) Use of an image analysis system to karyotype diploid alfalfa, Medicago sativa L. Journal of Heredity, 85, 18–22.
Diwan, N., Bauchan, G.R. & McIntosh, M.S. (1994) A core collection for the United States annual Medicago germplasm collection. Crop Science, 34, 279–285. https://doi.org/10.2135/cropsci1994.0011183X003400010051x
Diwan, N., McIntosh, M.S. & Bauchan, G.R. (1994) Methods of developing a core collection of annual Medicago species. Theoretical and Applied Genetics, 90, 755–761. https://doi.org/10.1007/BF00222008
Bauchan, G.R. & Hossain, M.A. (1996) Karyotypic analysis of C-banded chromosomes of diploid alfalfa: Medicago sativa ssp. caerulea and ssp. falcata and their hybrid. Journal of Heredity, 88, 533–537. https://doi.org/10.1093/oxfordjournals.jhered.a023152
Diwan, N., Cregan, P., Bauchan, G.R. & Bhagwat, A. (1997) Simple sequence repeat (SSR) DNA markers in alfalfa and perennial and annual Medicago species. Genome, 40, 887–895. https://doi.org/10.1139/g97-115
Bauchan, G.R. & Hossain, M.A. (1997) Identification of alfalfa chromosomes using Giemsa banding and image analysis techniques. Proceedings of the International Grasslands Congress. Winnipeg, Manitoba, Canada. June, pp. 61–62. https://doi.org/10.1093/jhered/89.2.191
Bauchan, G.R. & Hossain, M.A. (1997) Karyotypic analysis of C-banded Chromosomes of diploid alfalfa: Medicago sativa ssp. caerulea and ssp. falcata and their hybrid. Journal of Heredity, 88, 533–537. https://doi.org/10.1093/oxfordjournals.jhered.a023152
Turano, F. Dasher, R., Upadhyaya, A., Caldwell, C. & Bauchan, G.R. (1998) Characterization of glutamate dehydrogenase isoenzyme system in germinating soybean. Plant Science, 135, 137–148. https://doi.org/10.1016/S0168-9452(98)00082-X
Bauchan, G.R. & Hossain, M.A. (1999) Advances in alfalfa cytogenetics. In: One-hundred years of alfalfa genetics. The Alfalfa Genome. http://www.naaic.org/TAG/TAGpapers/Bauchan/advcytog.htm
Bauchan, G.R. (1999) Use of annual medics in sustainable agriculture systems. Proceedings of the EUCARPIA. Forage Researchers European Crop Society, Perugia, Italy, pp. 146–153.
Bauchan, G.R. & Hossain, M.A. (1999) Constitutive heterochromatic DNA polymorphisms in diploid Medicago sativa ssp. falcata. Genome, 40, 428–429. https://doi.org/10.1139/g99-038
Hossain, M.A. & Bauchan, G.R. (1999) Brief communication. Identification of B chromosomes using Giemsa banding in Medicago. Journal of Heredity, 90, 428–429. https://doi.org/10.1093/jhered/90.3.428
Kuykendall, L.D., Hashem, F.M., Bauchan, G.R., Devine, T.E. & Dadson, R.B. (1999) Symbiotic competence of Sinorhizobium fredii on twenty alfalfa cultivars of diverse dormancy. Symbiosis, 27, 1–16.
O'Neill, N.R. & Bauchan, G.R. (1999) Sources of resistance to Anthracnose in the annual Medicago core collection. Plant Disease, 84, 261–267. https://doi.org/10.1094/PDIS.2000.84.3.261
Skinner, D.Z., Bauchan, G.R. Auricht, G. & Hughes, S. (1999) Developing Core Collections for a large annual Medicago Collection. In: Johnson, R.C. & Hodgkin, T. (eds.). Core Collections of Today and Tomorrow. Rome, Italy, pp. 61–67.
Skinner, D.Z., Bauchan, G.R., Auricht, G. & Hughes, S. (1999) A method for the efficient management and utilization of large germplasm collections. Crop Science, 39, 1237–1242. https://doi.org/10.2135/cropsci1999.0011183X003900040046x
Bauchan, G.R. (2001) Annual Medics: What are they and how do they fit into forage and farming systems. Proceedings of the American Society of Agronomy. Charlotte, NC p. 428.
Bauchan, G.R. & Hossain, M.A. (2001) Distribution and characterization of heterochromatic DNA in the tetraploid ‘African’ alfalfa genome. Crop Science, 41, 1921–1926. https://doi.org/10.2135/cropsci2001.1921
Bauchan, G.R. & Hossain, M.A. (2001) A computerized image analysis system to characterize small plant chromosomes. Microscopy & Analysis, 48, 5–7.
Bauchan, G.R. & Greene, S.L. (2002) Status of the Medicago germplasm collection in the United States. International Plant Genetic Resources Institute. Plant Genetic Resources Newsletter, 129, 1–10.
Bauchan, G.R., Campbell, T.A. & Hossain, M.A. (2002) Chromosomal polymorphism as detected by C-banding patterns in ‘Chilean’ alfalfa germplasm. Crop Science, 42, 1291–1297. https://doi.org/10.2135/cropsci2002.1291
Campbell, T.A. & Bauchan, G.R. (2002) Organelle based molecular analysis of the genetic relatedness of cultivated alfalfa (Medicago sativa) to M. edgeworthii and M. ruthenica. Euphytica, 125, 51–58. https://doi.org/10.1023/A:1015796326675
Bauchan, G.R. Campbell, T.A. & Hossain, M.A. (2003) Comparative chromosome banding studies of non dormant alfalfa germplasm. Crop Science, 43, 2037–2042. https://doi.org/10.2135/cropsci2003.2037
O'Neill, N.R., Bauchan, G.R. & Samac, D.A. (2003) Reactions in the annual Medicago spp. Core germplasm collection to Phoma medicaginis. Plant Disease, 87, 557–562. https://doi.org/10.1094/PDIS.2003.87.5.557
Vandermark, G.J., Ariss, J.J., Bauchan, G.R., Larsen, R.C. & Hughes, T.J. (2006) Estimated genetic relationships among historical sources of alfalfa germplasm and selected cultivars with sequence related amplified polymorphism. Euphytica, 152, 9–16. https://doi.org/10.1007/s10681-006-9167-7
Robins, J.G., Luth, D., Campbell, T.A., Bauchan, G.R., He, C., Viands, D.R., Hansen, J.L. & Brummer, E.C. (2007) Genetic mapping of biomass production in tetraploid alfalfa. Crop Science, 47, 1–10. https://doi.org/10.2135/cropsci2005.11.0401
Robins, J.G., Bauchan, G.R. & Brummer, E.C. (2007) Genetic mapping forage yield, plant height, and regrowth at multiple harvests in tetraploid alfalfa (Medicago sativa L.). Crop Science, 47, 11–18. https://doi.org/10.2135/cropsci2006.07.0447
Bauchan, G.R. (2009) Alfalfa (Medicago sativa ssp. sativa (L.) L. & L.). In: Singh, R.J. (ed.) Genetic Resources, Chromosome Engineering, and Crop Improvement: Forage Crops. Vol. 5, Chapter 2. pp. 11–39. https://doi.org/10.1201/9781420047400.ch2
He, C., Xia, Z.L., Campbell, T.A. & Bauchan, G.R. (2009) Development and characterization of SSR markers and their use to assess genetic relationships among alfalfa germplasm. Crop Science, 49, 2176–2186. https://doi.org/10.2135/cropsci2007.04.0456
Carta, L.K., Bauchan, G.R., Hsu, C-Y & Yuceer, C.Y. (2010) Description of Parasitorhabditis mississippii, n. sp. (Nemata: Rhabditida) from Dendroctonus frontalis Zimmermann (Coleoptera: Scolytidae). Journal of Nematology, 42, 46–54.
Dowling, A.P.G., Bauchan, G.R., Beard, J.J. & Ochoa, R. (2010) SEM vouchers and genomic data from an individual specimen: maximizing the utility of delicate and rare specimens. Acarologia, 50, 479–485. https://doi.org/10.1051/acarologia/20101983
Gamliel-Atinsky, E., Freeman, S., Maymon, M., Belausov, E., Ochoa, R., Bauchan, G.R. Skoracka, A., Pena, J. & Palevsky, E. (2010) The role of eriophyoids in fungal pathogen epidemiology, mere association or true interaction? Experimental & Applied Acarology, 51, 191–204. https://doi.org/10.1007/978-90-481-9562-6_10
Jang, S.I., Lillehoj, H.S., Lee, S.H., Lee, K.W., Park, M.S., Bauchan, G.R., Lillehoj, E.P., Bertrand, F., Dupuis, L. & Deville, S. (2010) Immunoenhancing effects of Montanide ISA oil-based adjuvants on recombinant coccidian antigen vaccination against Eimeria acervulina infection. Veterinary Parasitology, 172, 221–228. https://doi.org/10.1016/j.vetpar.2010.04.042
Li, G., Lillehoj, H.S., Lee, K.Y., Lee, S.H., Park, M.S., Jang, S.I., Park, G.M., Bauchan, G.R., Gay, C.G., Ritter, D., Bautista, D. & Siragusa, G.R. (2010) Immunopathology and cytokine responses in commercial broiler chickens with Gangrenous dermatitis. Avian Pathology, 39, 255–264. https://doi.org/10.1080/03079457.2010.495382
Lim, H.-S., Varia, A.M., Bae, H., Bragg, J.N., Ruzin, S. E., Bauchan, G. R., Dienelt, M.M., Owens, R.A. & Hammond, J. (2010) Mutation of a chloroplast-targeting signal in Alternanthera mosaic virus TGB3 impairs cell-to-cell movement and eliminates long-distance virus movement. Journal of General Virology, 91, 2102–2115. https://doi.org/10.1099/vir.0.019448-0
Macarisin, D., Bauchan, G.R. & Fayer, R. (2010) Spinacia oleracear L. Leaf stomata harboring Cryptosporidium parvum oocysts: a potential threat for food safety. Applied and Environmental Microbiology, 76, 555–559. https://doi.org/10.1128/AEM.02118-09
Macarisin, D., Santin, M., Bauchan, G.R. & Fayer, R. (2010) Infectivity of Cryptosporidium arvum oocysts after storage of experimentally contaminated apples. Journal of Food Protection, 73, 1824–1829. https://doi.org/10.4315/0362-028X-73.10.1824
Macarisin, D., Wisniewski, M., Bauchan, G.R. & Samir. D. (2010) The biology of postharvest yeast biocontrol agents. I. Superoxide anion and hydrogen peroxide in the antagonist yeast-fruit interaction: a new role for reactive oxygen species in postharvest biocontrol. Applied & Environmental Microbiology, 58, 194–202. https://doi.org/10.1016/j.postharvbio.2010.07.008
Fisher, J.R., Skvarla, M.J., Bauchan, G.R., Ochoa, R. & Dowling, A.P.G. (2011) Trachymolgus purpureus sp. n., an armored snout mite (Acari: Bdellidae) from the Ozark highlands: morphology, development, and key to Trachymologus Berlese. ZooKeys, 125, 1–34. https://doi.org/10.3897/zookeys.125.1875
Menon, P., Ochoa, R., Bauchan, G.R., Joshi, S. & Ramamurthy, V. (2011) A new genus and species Mangalaus bkapus (Acari: Eriophyidae) from India. International Journal of Acarology, 37, 131–142. https://doi.org/10.1080/01647954.2010.497165
Ochoa, R., Beard, J.J., Bauchan, G.R., Kane, E.C., Dowling, A.P.G. & Erbe, E.F. (2011) Herbivore exploits chink in armor of host. American Entomologist, 57, 26–29. https://doi.org/10.1093/ae/57.1.26
Wen, Y., Wang, W., Feng, J., Luo, M.-C., Tsuda, K., Katagiri, F. Bauchan, G. & Xiao, S. (2011) Identification and utilization of a sow thistle powdery mildew as a poorly adapted pathogen to dissect post-invation non-host resistance mechanisms in Arabidopsis. Journal of Experimental Botany, 62, 2117–2129. https://doi.org/10.1093/jxb/erq406
Beard, J.J., Ochoa, R., Bauchan, G.R., Trice, M., Redford, A., Walters, T. & Mitter, C. (2012) Flat mites of the world. Part1: Raoiella and Brevipalpus. Identification Technology Program. www.idtools.org/id.mites/flatmites/
Beard, J.J., Ochoa, R. Bauchan, G.R., Welbourn, W.C., Pooley, C. & Dowling, A.P.G. (2012) External mouth-part morphology in the Tenuipalpidae (Tetranychoidea): Raoiella a case study. Experimental & Applied Acarology, 57, 227–255. https://doi.org/10.1007/s10493-012-9540-2
Konvipasruang, P., Chandrapatya, A., Amrine, J.W., Ochoa, R., Bauchan, G.R. & Pratt, P. (2012) A new species, Aceria neopaederiae (Acari: Eriophyidae), infesting Paederia foetida L. (Rubiaceae) in Thailand, Hong Kong and Singapore. Systematic & Applied Acarology, 17(2), 191–201. https://doi.org/10.11158/saa.17.2.5
Lakshman, D.K., Pandy, R., Kamo, K., Bauchan, G.R. & Mitra, A. (2012) Genetic transformation of Fusarium oxysporum f.sp. gladioli with Agrobacterium to study pathogenesis in Gladiolus. European Journal of Plant Pathology, 133, 729–738. https://doi.org/10.1007/s10658-012-9953-0
Lee, A.K., Hong, J., Bauchan, G.R., Park, S.H. & Joung, Y.H. (2012) Confirmation of hybrid origin of Cyrthanthus based on the sequence analysis of internal transcribed spacer. Scientia Horticulturae, 144, 153–160. https://doi.org/10.1016/j.scienta.2012.07.010
Macarisin, D., Patel, J., Bauchan, G.R., Giron, J.A. & Sharma, V.K. (2012) Role of curli and cellulose expression in adherence of Escherichia coli O157:H7 to spinach leaves. Foodborne Pathogens & Disease, 9, 160–167. https://doi.org/10.1089/fpd.2011.1020
Macarisin, D., O'Brien, C., Fayer, R., Bauchan, G.R. & Jenkins, M. (2012) Immunolocalization of beta and gama-giardin within the ventral disk in trophozoites of Giardia duodenalis using multiplex laser scanning confocal microscopy. Parasitology Research, 111, 241–248. https://doi.org/10.1007/s00436-012-2825-x
Nickle, D.A. & Bauchan, G.R. (2012) Low-temperature scanning electron microscopy to evaluate morphology and predation of Scolothrips sexmaculatus Pergande (Thysanoptera: Thripidae) against spider mites (Acari: Tetranychidae: Tetranychus spp.) American Entomologist, 58, 40–48. https://doi.org/10.1093/ae/58.1.0040
Oten, K.L.F., Bauchan, G.R., Frampton, J. & Hain, F.P. (2012) Biophysical characteristics of the stem and petiole surface of six hemlock (Tsuga) species and a hybrid: implications for resistance to Adelges tsugae. Botany, 90, 1170–1178. https://doi.org/10.1139/b2012-095
Roh, M.S. Bauchan, G.R. & Huda, M.S. (2012) The effect of biobased plastic resins containing chicken feather fibers on the growth and flowering of Begonia boliviensis. Horticulture Environment & Biotechnology, 53, 81–91. https://doi.org/10.1007/s13580-012-0118-z
Roh, M.S. Bauchan, G.R. & Huda, M.S. (2012) Physical and chemical properties of biobased plastic resins containing chicken feather fibers. Horticulture Environment & Biotechnology, 53, 72–80. https://doi.org/10.1007/s13580-012-0117-0
Roh, M.S., Bauchan, G.R., Murphy, C. & Bunce, J.A. (2012) The property and effect of bioplastic pots on the growth and developmental physiology of lily and begonia. Horticulture Environment & Biotechnology, 53, 467–476. https://doi.org/10.1007/s13580-012-1311-9
Shen, C., Luo, Y., Nou, X., Bauchan, G.R., Zhou, B., Wang, Q. & Millner, P. (2012) Enhanced inactivation of Salmonella and Pseudomonas biofilms on stainless steel by use of T-128, a fresh-produce washing aid, in chlorinated wash solutions. Applied & Environmental Microbiology, 78, 19, 6789–6798. https://doi.org/10.1128/AEM.01094-12
Vaira, A.M., Lim, H.-S., Bauchan, G.R., Owens, R.A., Natilla, A., Dienelt, M.M., Reinsel, M.D. & Hammond, J. (2012) Lolium latent virus (Alphaflexiviridae) coat proteins: expression and functions in infected plant tissue. Journal of General Virology, 93, 1814–1824. https://doi.org/10.1099/vir.0.042960-0
Yossa, N., Patel, J., Macarisin, D., Millner, P., Murphy, C., Bauchan, G.R. & Martin Lo, Y. (2012) Antibacterial activity of cinnamaldehyde and sporran against Escherichia coli 0157:H7 and Salmonella. Journal of Food Processing Preservation, 38, 749–757 (2014). https://doi.org/10.1111/jfpp.12026
Barnaby, J.Y., Kim, M., Bauchan, G.R., Bunce, J., Reddy, V. & Sicher, R.C. (2013) Drought responses of foliar metabolites in three maize hybrids differing in water stress tolerance. PLOS ONE, 8, 10, https://doi.org/10.1371/journal.pone.0077145.
Beard, J.J., Ochoa, R., Childers, C.C., Bauchan, G.R. & Shepard, M. (2013) Traveling with tea: A Tuckeralla's tale. Experimental & Applied Acarology, 59, 177–202. https://doi.org/10.1007/s10493-012-9627-9
Broadhurst, C.L., Bauchan, G.R., Murphy, C.A. Tang, Y.-T., Pooley, C. Davis, A.P. & Chaney, R.L. (2013) Accumulation of zinc and cadmium and localization of zinc in Picris divaricata. Environmental & Experimental Botany, 87, 1–9. https://doi.org/10.1016/j.envexpbot.2012.08.010
Fayer, R., Santin, M., Macarisin, D. & Bauchan, G.R. (2013) Adhesive-tape recovery combined with molecular and microscopic testing for the detection of Cryptosporidium oocysts on experimentally contaminated fresh produce and a food preparation surface. Parasitology Research, 112, 1567–1574. https://doi.org/10.1007/s00436-013-3305-7
Jang, C., Seo, E.-Y., Nam, J., Bae, J., Gim, Y.G., Kim, H.G., Cho, I.S., Lee, Z.-W., Bauchan, G.R., Hammond, J. & Lim, H.-S. (2013) Insights into Alternanthera mosaic virus TGB3 functions: interactions with Nicotiana benthamiana PSbO correlate with chloroplast vesiculation and veinal necrosis caused by TGB3 over-expression. Frontiers in Plant Science, 4(5), 1–15. https://doi.org/10.3389/fpls.2013.00005
Macarisin, D., Patel, J., Bauchan, G.R., Giron, J.A. & Ravishankar, S. (2013) Effect of spinach cultivar and bacterial adherence factors on survival of Escherichia coli O157:H7 on spinach leaves. Journal of Food Protection, 76, 1829–1837. https://doi.org/10.4315/0362-028X.JFP-12-556
Youssef, R.M., MacDonald, M.H., Brewer, E.P., Bauchan, G.R., Kim, K.-H. & Matthews, B.F. (2013) Ectopitic expression of AtPAD4 broadens resistance of soybean to soybean cyst and root-knot nematodes. BMC Plant Biology, 13, 67–78. https://doi.org/10.1186/1471-2229-13-67
Beard, J.J., Seeman, O.D. & Bauchan, G.R. (2014) Tenuipalpidae (Acari: Trombidiformes) from Casuarinaceae (Fagales). Zootaxa, 3778, 1–157. http://dx.doi.org/10.11646/zootaxa.3778.1.1
Bolton, S.J., Klompen, H., Bauchan, G.R. & Ochoa, R. (2014) A new genus and species for Nematalycidae (Acari: Endeostigmata). Journal of Natural History, 48(23–24), 1359–1373. https://doi.org/10.1080/00222933.2013.859318
Vega, F.E., Simpkins, A., Bauchan, G.R., Infante, F., Kramer, M. & Land, M.F. (2014) On the eyes of male coffee berry borers as rudimentary organs. PLOS ONE, 9, e85860. https://doi.org/10.1371/journal.pone.0085860
Beard, J.J., Ochoa, R., Braswell, W. & Bauchan, G.R. (2015) Brevipalpus phoenicis (Geijskes) Species Complex: a Closer Look. Zootaxa, 3944(1), 1–67. https://doi.org/10.11646/zootaxa.3944.1.1
Bolton, S.J., Bauchan, G.R., Ochoa, R., Pooley, C. & Klompen, H. (2015) The role of the integument with respect to different modes of locomotion in the Nematalycidae (Endeostigmata). Experimental & Applied Acarology, 65, 149–161. https://doi.org/10.1007/s10493-014-9857-0
Bolton S.J., Bauchan, G.R., Ochoa, R. & Klompen, H. (2015) A novel fluid-feeding mechanism for microbivory in the Acariformes (Arachnida: Acari). Arthropod Structure and Development, 44(4), 313–325. https://doi.org/10.1016/j.asd.2015.04.009
Castro, E.B., Ochoa, R., Feres, R.J.F., Beard, J.J. & Bauchan, G.R. (2015) Reinstatement of the genus Colopalpus Pritchard & Baker (1958) and re-description of Colopalpus matthyssei P. & B. (1958), the type species of the genus (Acari, Tenuipalpidae). International Journal of Acarology, 41(4), 310–328. https://doi.org/10.1080/01647954.2015.1031276
Favret, C., Tzaud, M., Erbe, E.F., Bauchan, G.R. & Ochoa, R. (2015) An adhesive collophore may help direct the springtail jump. Annals of the Entomological Society of America, 108, 1–6. https://doi.org/10.1093/aesa/sav078
Fisher, J. R., Fisher, D.M., Nelson, W.A., O'Neill, J.C., Skvarla, M.J., Ochoa, R., Bauchan, G.R., Radwell A.J. & Dowling, A.P.G. (2015) Torrenticola trimaculata n. sp. (Parasitengona: Torrenticolidae), a three-spotted water mite from eastern North America: taxonomic history, species delimitation, and survey of external morphology. Acarologia, 55(1), 71–116. https://doi.org/10.1051/acarologia/20152155
Rezende, J.M., Lofego, A.C., Ochoa, R. & Bauchan, G.R. (2015) New species of Daidalotarsonemus and Excelsotarsonemus (Acari, Tarsonemidae) from the Brazilian rainforest. ZooKeys, 475, 1–36. https://doi.org/10.3897/zookeys.475.8827
Castro, E.B., Kane, E.C., Feres, R.J.F., Ochoa, R. & Bauchan, G.R. (2016) Definition of Tenuipalpus sensu stricto (Acari: Tenuipalpidae), with redescription of Tenuipalpus caudatus (Duges) and description of a new species from Costa Rica. International Journal of Acarology, 42, 106–126. https://doi.org/10.1080/01647954.2015.1130941
Castro, E.B., Feres, R.J.F., Ochoa, R. & Bauchan, G.R. (2016) A new species of Tenuipalpus sensu stricto (Acari: Tenuipalpidae) from Brazil, with ontogeny and a key to the known species. Zootaxa, 4088(3), 355–378. https://doi.org/10.11646/zootaxa.4088.3.3
Camerik, A.M., Magowski, W., Hawkes, P., Ueckermann, E.A., Ochoa, R. & Bauchan, G.R. (2016) A new species of Zambedania (Acari: Heterostigmata: Pygmephoridae) from Two Rivers Platinum Mine in South Africa and notes on the life-cycle of the genus. Zoological Studies, 55(11), 1–21. http://dx.doi.org/10.6620/ZS.2016.55-11
Hernandes, F.A., Mironov, S.V., Bauchan, G.R. & Ochoa, R. (2016) A new asymmetrical feather mite of the genus Michaelia Trouessart, 1884 (Astigmata: Freyanidae) from the Neotropical Cormorant, Phalacrocorax brasilianus (Pelecaniformes). Acarologia, 56, 45–61. https://doi.org/10.1051/acarologia/20162187
Hernandes, F.A., Skvarla, M.J., Fisher, J.R., Dowling, A.P.G., Ochoa, R., Ueckermann, E.A. & Bauchan, G.R. (2016) Catalogue of snout mites (Acariformes: Bdellidae) of the world. Zootaxa, 4152, 1–83. https://doi.org/10.11646/zootaxa.4152.1.1
Castro, E.B., Ramos, F.A.M., Feres, R.J.F., Ochoa, R. & Bauchan, G.R. (2017) Redescription of Tenuipalpus heveae Baker (Acari: Tenuipalpidae) and description of a new species collected on rubber tree from Amazonia, Brazil. Acarologia, 57, 421–458. https://doi.org/10.1051/acarologia/20174166
Chetverikov, P.E., Amrine, J., Bauchan, G.R., Ochoa, R., Sukhareva, S.I. & Vishnyakov, A.E. (2017) Supplementary description of Novophytoptus stipae Keifer 1962 (Acariformes, Eriophyoidea) with LT-SEM observation on mites from putatively conspecific populations: cryptic speciation or polyphagy of novophytoptines on phylogenetically remote hosts? Systematic & Applied Acarology, 22(2), 253–270. https://doi.org/10.11158/saa.22.2.9
Hernandes, F.A., OConnor, B.M., Bauchan, G.R. & Ochoa, R. (2017) A new species of Proctophyllodes Robin, 1868 (Acari: Proctophyllodidae) from two tanagers of the genus Piranga Vieillot (Passeriformes: Cardinalidae) from North America. Journal of Natural History, 51(41–42), 2407–2416. https://doi.org/10.1080/00222933.2017.1381772
Hernandes, F.A., Bauchan, G.R. & Ochoa, R. (2017) New and little known feather mites (Acariformes: Astigmata) analysed with low-temperature scanning electron microscopy. International Journal of Acarology, 43, 499–517. https://doi.org/10.1080/01647954.2017.1367032
Metz, M.A., Miller, D.R., Dickey, A.M., Bauchan, G.R., Ochoa, R., Skvarla, M. & Miller, G. (2017) Rediscovering digitules in Aphidomorpha and the question of homology among Sternorrhyncha (Insecta, Hemiptera). ZooKeys, 683, 39–50. https://doi.org/10.3897/zookeys.683.10100
Welbourn, W.C., Beard, J.J., Bauchan, G.R. & Ochoa, R. (2017) Description of two species of Tenuipalpus (Acari: Trombidiformes) from succulent plants. International Journal of Acarology, 43(2), 112–136. https://doi.org/10.1080/01647954.2016.1255253
De Araujo, M.S., Bichuette, M.E., Bauchan, G.R., Ochoa, R. & Feres, R.J. (2018) A new species of cave dwelling Neocarus (Acari: Opilioacaridae) from Bahia state, Brazil, with remarks on taxonomic characters. Zootaxa, 4402,(2), 303–322. https://doi.org/10.11646/zootaxa.4402.2.4
Bassini-Silva, R., de Castro J.F., Maturano, R., Muñoz-Leal, S., Ochoa, R., Bauchan, G.R. Labruna, M.B., Barros-Battesti, D.M. (2018) Blankaartia sinnamaryi (Trombidiformes: Trombiculidae) parasitizing birds in southeastern Brazil, with notes on Rickettsia detection. Revista Brasileira de Parasitologia Veterinaria, 27, 354–362. https://doi.org/10.1590/S1984-296120180057
Bassini-Silva R., de Castro J.F., Muñoz-Leal, S., Maturano, R., Welbourn C.W., Ochoa, R., Bauchan, G.R. & Barros-Battesti, D.M. (2018) A new species of the genus Eutrombicula Ewing, 1938 (Trombidiformes: Trombiculidae) and new records for the species Eutrombicula batatas (Linnaeus, 1758) in Brazil. Acarologia, 58,(4), 976–986. https://doi.org/10.24349/acarologia/20184304
Beard, J.J., Ochoa, R., Bauchan, G.R., Pooley, C. & Dowling, A.P.G. (2018) Raoiella of the world (Trombidiformes: Tetranychoidea: Tenuipalpidae). Zootaxa, 4501, 1–302. https://doi.org/10.11646/zootaxa.4501.1.1
Bolton, S.J., Bauchan, G.R., Chetverikov, P.E., Ochoa, R. & Klompen, H. (2018) A rudimentary sheath for the smallest of ‘biting’ chelicerae: a precursor to the stylet sheath of Eriophyoidea (Acariformes). International Journal of Acarology, 44(8), 374–381. https://doi.org/10.1080/01647954.2018.1488274
Castro, E.B., Beard, J.J., Ochoa, R., Bauchan, G.R. & Feres, R. (2018) Two new species of Tenuipalpus sensu stricto (Acari: Tenuipalpidae) from Brazil, with a discussion on the ontogeny of leg setae. Zootaxa, 4540(1), 178–210. https://doi.org/10.11646/zootaxa.4540.1.12
Childers, C.C., de Lillo, E., Bauchan, G.R., Rogers, M.E., Ochoa, R. & Robinson, C. (2018) External morphology of the mouthparts and observations on behavior of Tuckerella japonica on Camellia sinensis in the continental USA. Experimental & Applied Acarology, 74, 55–71. https://doi.org/10.1007/s10493-017-0204-0
Gulbronson, C., Mowery, J.D., Pooley, C., Ochoa, R., Bolton, S.J. & Bauchan G.R. (2018) Three-Dimensional Printing of Agriculturally Important Mites Generated from Confocal Microscopy. Microscopy & Microanalysis, 24, 1360–1361. https://doi.org/10.1017/S1431927618007286
de Castro J.F., Bassini-Silva, R., Mendoza-Roldan, J.A., Muñoz-Leal, S., Hingst-Zaher, E., Ochoa, R., Bauchan, G.R. & Barros-Battesti, D.M. (2018) A contribution to the knowledge of Quadraseta brasiliensis Goff and Gettinger, 1989 (Trombidiformes: Trombiculidae), with description of the deutonymph instar. Acarologia, 58, 442–456. https://doi.org/10.24349/acarologia/20184252
Klimov, P.B., OConnor, B.M., Chetverikov, P.E., Bolton, S., Pepato, A., Mortazavi, A.L., Tolstikov, A.V., Bauchan, G.R. & Ochoa, R. (2018) Comprehensive phylogeny of acariform mites (Acariformes) provides insights on the origin of the four-legged mites (Eriophyoidea), a long branch. Molecular Phylogenetics & Evolution, 119, 105–117. https://doi.org/10.1016/j.ympev.2017.10.017
Ramsey, R., Gulbronson, C., Mowery, J., Ochoa, R., VanEngelsdorp, D. & Bauchan, G.R. (2018) A multimicroscopy approach to discover the feeding site and host tissue consumed by Varroa destructor on host Honey Bees. Microscopy & Microanalysis, 24, 1258–1259. https://doi.org/10.1017/S1431927618006773
Rezende, J.M., Lofego, A. Gulbronson, C., Bauchan, G.R. & Ochoa, R. (2018) Review of the genus Ceratotarsonemus DeLeon, 1956 (Acari: Prostigmata: Tarsonemidae), with description of a new species from the Amazon forest. Zootaxa, 4483(2), 271–294. https://doi.org/10.11646/zootaxa.4483.2.3
Otero-Colina, G., Ochoa, R., Amrine, J., Hammond, J., Jordan, R. & Bauchan, G.R. (2018) Eriophyoid mites found on healthy and Rose Rosette Diseased roses in the United States. Journal of Environmental Horticulture, 36(4), 146–153. https://doi.org/10.24266/0738-2898-36.4.146
Bassini-Silva R., de Castro J.F., Hernandes F.A., Ochoa, R., Bauchan, G.R., Dowling, A.P.G. & Barros-Battesti D.M. (2019) Dermatitis in humans caused by Ornithonyssus bursa (Berlese 1888) (Mesostigmata: Macronyssidae) and new records from Brazil. Revista Brasileira de Parasitologia Veterinaria, 28(1), 134–139. https://doi.org/10.1590/S1984-296120180097
Ramsey, S., Ochoa, R., Bauchan, G.R., Gulbronson, C., Mowery, J., Cohen, A., Lim, D., Joklik, J., Cicero, J., Ellis, J., Hawthornem, D.& vanEngelsdorp, D. (2019) Varroa destructor (Varroidae) feeds primarily on honeybee fat body tissue not hemolymph. Proceedings of National Academy of Sciences of the United States of America, 116(5), 1792–1801. https://doi.org/10.1073/pnas.1818371116
Ueckermann, E.A., Ochoa, R., Bauchan, G.R. & Neser, S. (2019) An amazing sub-cambium flat mite from South Africa (Acari: Trombidiformes: Tenuipalpidae). Acarologia, 59(4), 507–530. https://doi.org/10.24349/acarologia/20194351
Azevedo, L.H., Moreira, M.F.P., Pereira, G.G., Borges, V., de Moraes, G.J., Inomoto, M.M., Vicente, M., de Siqueira Pinto, M., Peres, L.P., Rueda-Ramírez, D., Carta, L., Meyer, S.L.F., Mowery, J., Bauchan, G.R., Ochoa, R. & Palevsky E. (2020) Combined releases of soil predatory mites and provisioning of free-living nematodes for the biological control of root-knot nematodes on ‘Micro Tom tomato.’ Biological Control, 146, 1–10. https://doi.org/10.1016/j.biocontrol.2020.104280
Carrillo, D., Cruz, L.F., Revynthi, A.M., Duncan, R.E., Bauchan, G.R., Ochoa, R., Paul E. Kendra, P.E. & Bolton, S. (2020) Detection of the Lychee Erinose Mite, Aceria litchi (Keifer) (Acari: Eriophyidae) in Florida, USA: a comparison with other alien populations. Insects, 11, 235, 1–12. https://doi.org/10.3390/insects11040235https://doi.org/10.3390/insects11040235
Liu, M., Yi, T.-C., Gulbronson, C., Bauchan, G.R. & Ochoa, R. (2020) Ontogenetic and morphological studies on Tetranychus Canadensis (Acari: Tetranychidae) Zootaxa, 4857(1), 215–250. https://doi.org/10.11646/zootaxa.4857.1.11
Roy, A., Stone, A.L., Otero-Colina, G., Brlansky, R.H., Ochoa, R., Bauchan, G.R., Schneider, W.L., Nakhla, M.K., & Hartung, J.S. (2020) Reassortment of RNA2 genome creates stable lineages among strains of Orchid fleck virus infecting citrus in Mexico. Phytopathology, 110, 106–120. https://doi.org/10.1094/PHYTO-07-19-0253-FI
Solo. K., Collins, S.B., Shires, M.K., Ochoa, R., Bauchan, G.R., Schneider, L.G., Henn, A., Jacobi, J.C., Williams-Woodward, J.L., Hajimorad, M.R., Hale, F.A., Wilkerson, J.B., Windham, A.S., Ong, K.L. & Windham, M.T. (2020) A Survey of Rose rosette virus and Eriophyid mites associated with roses in the Southeastern United States. Horticultural Science, 55(8), 1–7. https://doi.org/10.21273/HORTSCI14653-20
Sousa, A.S.G., Rezende, J.M., Lofego, A.C., Ochoa, R., Bauchan, G.R., Gulbronson, C. & Oliveira, A.R. (2020) Two new species of Tarsonemus (Acari Tarsonemidae) from Bahia, Brazil. Systematic & Applied Acarology, 25(6), 986–1012. https://doi.org/10.11158/saa.25.6.4
Combita-Heredia O., Gulbronson C.G., Ochoa, R., Quintero-Gutierrez, E.J., Bauchan, G.R. & Klompen H. (2021) Size, shape, and direction matters: matching secondary genital structures in male and female mites using multiple microscopy techniques and 3D modeling. PLOS ONE, 16(8), 001–019. https://doi.org/10.1371/journal.pone.0254974
Di Palma, A., Beard, J.J., Bauchan, G.R., Ochoa, R., Seeman, O. & Kitajima, E.W. (2021) Dorsal setae in Raoiella (Acari: Tenuipalpidae): their functional morphology and implication in fluid secretion. Arthropod Structure & Development, 60, 1–10. https://doi.org/10.1016/j.asd.2020.101023
