Three new species of the subgenus Pergalumna (Pergalumna)—P. (P.) chamaensis sp. nov., P. (P.) henriensis sp. nov. and P. (P.) parabifissurata sp. nov.—are described, based on adults collected from northern, northwestern and western Venezuela. Pergalumna (P.) chamaensis differs from its related species P. (P.) andicola and P. (P.) anellata by the morphology of the rostrum and the length of the lamellar seta. Pergalumna (P.) henriensis differs from its related species P. (P.) hypergranulosa and P. (P.) obsidiana by the larger body size, the morphology of the rostral region, and the body sculpturing. Pergalumna (P.) parabifissurata differs from the related species P. (P.) bifissurata by the morphology of the rostral region and the size of the notogastral porose areas. An identification key to the known species of Pergalumna from the Neotropical region is presented.