Proprioseiopsis asetus and Amblyseius swirskii are excellent natural enemies of plant-feeding mites and small pests. In order to clarify the safety of the acaricide spirodiclofen to the predatory mites P. asetus and A. swirskii, here, we investigated the toxicity of the acaricide on the pest mite T. urticae (nymphs and adult females) and on both species of predatory mites, P. asetus and A. swiskii (eggs, nymphs, and female adults) by using the spray, slide-dip, and leaf residual toxicity methods, compared the selective toxicity and assessed the safety to these natural enemies. The results demonstrated that the corrected mortality in P. asetus and A. swirskii female adults was <5% at recommended spirodiclofen concentrations in the spray method. In the leaf residual toxicity method, P. asetus and A. swirskii egg-hatching rates did not differ significantly from the pure water control group. In the slide-dip method, spirodiclofen lethal concentration 50 (LC₅₀) in P. asetus and A. swirskii female adults was noted to be 9,494.875 and 3,064.187 mg·L^–1, respectively. In the spray method, spirodiclofen LC₅₀ in P. asetus and A. swirskii nymphs was found to be 4,292.664 and 1,081.609 mg·L^–1, respectively, and that in T. urticae female adults and nymphs was 55.189 and 40.862 mg·L^–1, respectively. Spiroclofen selective toxicity ratios (STRs) in P. asetus female adults–T. urticae and P. asetus nymphs–T. urticae were 172.013 and 105.053, respectively, indicating that spiroclofen has a high positive selectivity toward the pest T. urticae and its natural enemy P. asetus. In contrast, spiroclofen STRs in A. swirskiis female adults–T. urticae and A. swirskiis nymphs–T. urticae were 55.522 and 26.470, respectively, indicating spiroclofen has a moderate positive selectivity toward the pest T. urticae and its natural enemy A. swirskii. In the safety evaluation, the spirodiclofen safety factor for P. asetus female adults and nymphs was 158.248–237.37 and 71.544–107.317, respectively, whereas that for A. swirskii female adults and nymphs was 50.070–76.605 and 18.027–27.040 respectively. All spirodiclofen safety factor values were much higher than 5, indicating that spirodiclofen is highly safe for P. asetus and A. swirskii; in particular, spirodiclofen is safer for P. asetus than for A. swirskii.