Amyris roseomaculata , a new species from the state of Durango, Mexico, is described and illustrated. The new species is morphologically similar to A. rekoi Blake because both have 5-merous flowers, unifoliolate leaves, and leaflets and petioles are similar in length. However, A. roseomaculata is differentiated from that species due to the shape of calyx lobes and nectary disc, and the ovary clearly differentiated from the nectary disc. In addition, A. roseomaculata is known only from Durango, Mexico, while A. rekoi is distributed only in the state of Oaxaca. A comparative table is included as well as images and a distribution map of the two species.