The firefly holdings of the Fernando de Zayas Collection, located in Havana, Cuba, is reported as well as the updated taxonomic status of these species. This is an important collection to the study of the Lampyridae of Cuba and remains one of the major insect collections in Cuba. The lampyrid holdings in this collection include the holotype of Alecton x-nigra Zayas 1988, and one paratype each of Callopisma maestro Mutchler 1923 and Callopisma monticola Mutchler 1923. Data gathered from this collection verifies that the lampyrid genera Robopus and Callopisma are the most speciose. Literature based on outdated taxonomy put the genus Photinus as most speciose genus of firefly in Cuba.