Translator Disclaimer
12 August 2015 Stable hydrogen isotopes identify leapfrog migration, degree of connectivity, and summer distribution of Golden Eagles in eastern North America
Author Affiliations +
Abstract

Knowledge of the distribution and movements of populations of migratory birds is useful for the effective conservation and management of biodiversity. However, such information is often unavailable because of the difficulty of tracking sufficient numbers of individuals. We used more easily obtained feather stable hydrogen isotope ratios (δ2H) to predict the summer grounds of the small, threatened, and migratory population of Golden Eagles (Aquila chrysaetos) in eastern North America. We then identified summer locations and the extent of migratory connectivity for this population. We collected δ2H (δ2Hf), stable carbon isotope (δ13C), and stable nitrogen isotope (δ15N) data from the body feathers of 47 juvenile, subadult, and adult Golden Eagles. Values of δ13C and δ15N suggested that all but 2 birds obtained food from terrestrial-based food webs and therefore that δ2H data were appropriate for inferring the geographic region of molt for the majority of birds. There was relatively large interfeather variation in the δ2H values of subadults vs. adults, suggesting that these groups molted at different times and places. The most negative δ2Hf values from birds with known summering grounds exhibited (1) a negative correlation with their summering latitude, and (2) a positive correlation with amount-weighted δ2H values of May–August precipitation at the summer location. These data validate the use of δ2Hf values for inferring the summer locations of Golden Eagles of unknown origin. Likelihood-of-origin maps derived from δ2Hf values revealed that (1) the majority of birds spent the breeding season in central Québec and Labrador, and (2) birds that wintered at southern latitudes, from approximately northern Alabama to southwestern Virginia, migrated about twice the distance of birds that wintered at northern latitudes, from Pennsylvania to New York. We observed a positive relationship between δ2Hf values and the latitude of the wintering location, which, along with the likelihood-of-origin maps, revealed moderate patterns of leapfrog migration and migratory connectivity.

INTRODUCTION

Knowledge of the distribution of migratory animals throughout their annual cycle is critical to identifying the ecological and evolutionary processes that shape their population dynamics, as well as to developing effective conservation measures (e.g., Bauer and Hoye 2014). For example, the location and quality of habitat that migratory birds occupy during winter can profoundly affect their fitness and fecundity during the summer breeding season (e.g., Norris et al. 2004) and may have important demographic consequences (e.g., Harrison et al. 2013). Migratory connectivity, the degree to which individuals from the same breeding area migrate to the same wintering area (Webster et al. 2002), however, remains difficult to assess for many bird species because of challenges associated with tracking sufficient numbers of individuals for population-level inference. Remote telemetry systems provide precise details on year-round location (e.g., Miller et al. 2014), but the devices are costly and often challenging to deploy. Similarly, mark–recapture and light-level geolocation approaches can provide insights into migratory connectivity (e.g., Fort et al. 2012, Stanley et al. 2015), but suffer from low recapture rates for many species (e.g., Bridge et al. 2013).

Stable hydrogen isotope ratios (δ2H) help to overcome many of these challenges and have thus become an important tool for inferring the geographic origins of migratory wildlife (e.g., Hobson 1999, Bowen et al. 2005), including patterns of avian migratory connectivity (e.g., Kelly et al. 2002, Clegg et al. 2003, Smith et al. 2003, Paxton et al. 2007, Hobson et al. 2014, Rushing et al. 2014). The basis of this approach is that the δ2H values in animal tissues are derived from the δ2H values of drinking water and diet. The δ2H values of water and diet originate from the δ2H values of precipitation (δ2Hp), which exhibit pronounced continental-scale gradients, with lower values at higher latitudes and elevations (Bowen 2010). The relationship between the δ2H values of precipitation and animal tissue is modified by environmental and biosynthetic isotopic discrimination. Positive relationships between the δ2H values of feather keratin (δ2Hf) and amount-weighted growing-season precipitation δ2Hp values in multispecies avian datasets suggest that isotopic discrimination predictably influences δ2Hf values across species, age classes, and years of collection (e.g., Bowen et al. 2005, Lott and Smith 2006, van Dijk et al. 2014). However, intra- and interspecific variation in such relationships can also limit δ2H-based inferences of origin (e.g., Smith and Dufty 2005, Hobson et al. 2012, De Ruyck et al. 2013, Hallworth et al. 2013).

Eagles (Accipitridae) have immense cultural and ecological significance worldwide (Tingay and Katzner 2010, Watson 2010). In eastern North America there is a small (n ≅ 5,000) population of migratory Golden Eagles (Aquila chrysaetos; Katzner et al. 2012a, Dennhardt et al. 2015). These migrants breed in remote areas of eastern Canada, winter in the United States east of the Mississippi River, and are geographically distinct from the much larger population of Golden Eagles in the western United States (Kochert et al. 2002, Nielson et al. 2014; Figure 1). Federal, state, and provincial regulations have long afforded Golden Eagles strong protection in the United States and Canada. Nevertheless, decision-makers are increasingly concerned about eastern Golden Eagles because of their small population size and potential susceptibility to threats (Katzner et al. 2012a). Adult Golden Eagles are known to exhibit interyear fidelity to particular summer and winter regions (Brodeur et al. 1996), but it is unknown whether adults from similar breeding locations migrate to geographically similar or distinct winter locations. For example, despite nearly a century of mark–recapture efforts, only 1 of the 1,144 encounter records for Golden Eagles in the U.S. Geological Survey's Bird Banding Laboratory dataset from 1926 to 2013 is suitable for assessing migratory connectivity of eastern Golden Eagles.

FIGURE 1.

Range of Golden Eagles in eastern North America (Kochert et al. 2002, Wheeler 2007, Cadman et al. 2009, Katzner et al. 2012a, Morneau et al. 2015), along with the locations of the individuals from the 4 winter populations that were sampled for connectivity analyses. Note that some symbols represent the location of multiple birds.

i0010-5422-117-3-414-f01.tif

To our knowledge, no prior published isotopic studies of Golden Eagles exist. Thus, it is uncertain how well δ2Hf values may record the geographic origins of these birds. Several considerations need to be evaluated before using isotopic data to infer eagle movements. First, it is not known whether the positive relationship that exists between δ2Hf and δ2Hp values for nestlings of 12 species of raptors of known origin from throughout North America (Lott and Smith 2006) is appropriate for the conversion of δ2Hf to δ2Hp values for (1) nonnestling raptors and (2) eastern Golden Eagles. Second, the complex patterns of feather replacement by Golden Eagles could complicate the use of δ2Hf for inferring geographic origin. Unlike smaller birds that molt once per year, usually following breeding, larger birds, such as Golden Eagles, molt their entire complement of feathers over the course of several years (Pyle 1997, Rohwer et al. 2009). Molting by Golden Eagles is thought to be more common during the summer breeding season, but it can occur at other times of the year (Jollie 1947, Bloom and Clark 2001). If correct, this suggests that δ2H data from a single feather may not be indicative of the summering location of a bird. Furthermore, substantial variation in δ2H values has been observed along the length of flight feathers from Bald Eagles (Haliaeetus leucocephalus), probably because the growth of flight feathers occurs over several weeks (Wassenaar 2008). Thus, flight feathers of Golden Eagles are likely to show considerable variation in isotopic values even within the same individual.

Factors such as intraspecific competition, time and energy costs associated with movement, gradients in habitat quality, and historical or genetic constraints are often invoked to explain patterns of strong migratory connectivity between the wintering and summering grounds of birds (Alerstam et al. 2003). However, there is insufficient understanding of the biology and ecology of Golden Eagles with which to make such specific hypotheses. Regardless of cause, an understanding of the summer locations and migration patterns of Golden Eagles has conservation implications. If migratory connectivity is strong, then eastern Golden Eagles from distinct breeding areas will be seasonally linked to distinct wintering regions. Strong connectivity would suggest that Golden Eagles have small, vulnerable subpopulations with limited ability to adapt to environmental changes (e.g., Both and Visser 2001). In contrast, if migratory connectivity is weak (i.e. populations are well mixed between their summer and winter regions), then any impacts of environmental change will spread throughout the population (e.g., Webster et al. 2002). We obtained δ13C, δ15N, and δ2H values from the feathers of live-captured and museum specimen Golden Eagles to: (1) assess the potential of using these data to identify the summer locations of eastern Golden Eagles; (2) identify the summer locations used by Golden Eagles in eastern Canada; and (3) determine the degree of migratory connectivity.

METHODS

Capture of Live Birds, Deployment of Telemetry Units, and Feather Collection

Golden Eagles were trapped from November to March between 2006 and 2014, from Alabama to New York, USA, with cannon nets on wintering grounds or with a bow net while on migration (Bloom et al. 2007;  Supplementary Material Table S1, Figure 1). Each bird was outfitted with a solar-recharged 45 g or 100 g ARGOS-GPS (Microwave Telemetry, Columbia, Maryland, USA) or 70 g or 95 g GPS–GSM (Cellular Tracking Technologies, Somerset, Pennsylvania, USA) telemetry unit with a Teflon ribbon (Bally Ribbon Mills, Bally, Pennsylvania, USA) harness in a backpack style (Fuller et al. 2005). Units weighed <3% of bird body mass. GSM units stored data collected outside GSM coverage and only transmitted data when birds were within GSM coverage (at latitudes lower than ~50°N). The age of each bird was determined using molt patterns (McCollough 1989, Bloom and Clark 2001), and birds were characterized as juvenile (first or second year of life; HY or 2Y), subadult (third or fourth year of life; 3Y or 4Y), or adult (older than fourth year of life; >4Y). The sex of each bird was determined genetically (Fridolfsson and Ellegren 1999). Prior to release we collected body feathers from each bird for isotopic analysis. We obtained between 3 and 7 body feathers for 14 birds, 2 body feathers for 28 birds, and a single body feather from 2 birds. We primarily analyzed body feathers because they are relatively small and thus likely to grow more quickly, and are therefore less likely to reflect temporal and spatial variation in isotopic values than flight feathers. However, on 3 occasions we collected <0.5 cm2 clippings from near the proximal end of in-sheath (i.e. actively growing) feathers obtained on the wintering grounds, as a reference for wintering δ2Hf values (Edelstam 1984).

The telemetry units collected GPS data at regular (30 s to 2 hr) intervals. We defined migration periods as occurring when birds initiated continuous north- or southbound movements along the primary axis of migration (Miller 2012); all other data were either from summer or winter grounds. We categorized the telemetry data for each bird into seasons and then calculated the centroid of the winter and summer grounds using the Spatial Analyst tool Zonal Geometry (ArcGIS 10.1; ESRI, Redlands, California, USA). We obtained centroids for birds with at least 20 days of telemetry locations from each season. We had fewer summer centroids because GSM units only transmitted data when birds were within GSM coverage. Thus, if birds died or units failed before data retrieval we could not calculate summer centroids for those birds. Also, several birds were captured during northbound migration from unknown wintering grounds ( Supplementary Material Table S1). We were able to calculate winter centroids for less than half of the birds. However, because the latitudes and longitudes of the winter centroids were strongly positively correlated with trapping locations for birds captured during winter (r = 0.93 and 0.97, respectively), we used the latitude and longitude of trapping locations when assigning birds to winter populations (see below). There were sufficient telemetry data to identify the summer grounds for 6 juvenile, 7 subadult, and 7 adult birds ( Supplementary Material Table S1), but only adults were inferred to have established territories based on their restricted summer movements.

Sampling of Museum Specimens

To increase our sample sizes, we obtained single body feathers from 3 specimens of eastern Golden Eagles housed in the Division of Birds collection at the Smithsonian Museum of Natural History. The age of each bird was determined as above for the live birds. We used collection date and age to infer information about summer or winter location of each bird ( Supplementary Material Table S1).

Isotopic and Geospatial Analysis

Between 2 and 3 subsamples were cut along the length (from tip to base) of each feather (Wassenaar and Hobson 2006). These subsamples were cleaned and dried as in Coplen and Qi (2012). Approximately 1 mg of each cleaned subsample of feather was analyzed for δ13C and δ15N values using a Carlo Erba NC2500 elemental analyzer (CE Instruments, Milano, Italy) interfaced with a ThermoFinnigan Delta V+ isotope ratio mass spectrometer (IRMS; ThermoFisher Scientific, Bremen, Germany) at the Central Appalachians Stable Isotope Facility (CASIF) at the Appalachian Laboratory (Frostburg, Maryland, USA). The δ13C and δ15N data were normalized to VPDB and AIR, respectively, using a 2-point normalization curve with internal standards calibrated against USGS40 and USGS41. The among-run analytical precision (1σ) of an internal keratin standard (porcine hair and skin; product K3030, Spectrum Chemical Manufacturing Corporation, New Brunswick, New Jersey, USA) analyzed alongside these samples was 0.11‰ for both δ13C and δ15N.

We measured δ2H values of nonexchangeable hydrogen in feather keratin using a comparative equilibration approach (Wassenaar and Hobson 2003). Approximately 0.2 mg of each cleaned subsample, as well as international standards (USGS42 and USGS43; Coplen and Qi 2012) and the internal keratin standard, was exposed to ambient air for >72 hr for equilibration of exchangeable hydrogen in keratin (Wassenaar and Hobson 2003). Samples were then placed in a zero-blank autosampler (Costech Analytical, Valencia, California, USA) and analyzed for δ2H values using a ThermoFinnigan high temperature conversion elemental analyzer interfaced with a ThermoFinnigan Delta V+ IRMS at CASIF. The δ2H data were normalized to VSMOW-SLAP using a 2-point normalization curve with USGS42 and USGS43 (Coplen and Qi 2012), whose δ2H values of nonexchangeable hydrogen are −78.5‰ and −50.3‰, respectively. The long-term analytical precision (1σ) of these standards at CASIF is 2.1‰ and 2.0‰, respectively. Our δ2Hf values spanned a larger range (−165.5‰ to −25.7‰) than do USGS42 and USGS43, but prior studies suggest that linear extrapolation of normalization relationships for δ2H values has minimal influence on values within ~100‰ of the range of the standards used for normalization (Kelly et al. 2009, Wiley et al. 2012). The long-term accepted δ2H value of the internal keratin standard at CASIF is −59.5 ± 2.3‰.

Prior studies have used δ2H values of growing-season precipitation or mean annual precipitation when developing transfer functions between δ2Hf and δ2Hp values for geospatial analysis (Bowen et al. 2005, Hobson 2011). However, the specific months of precipitation that most strongly influence the food webs supporting many birds (Hobson et al. 2012), including Golden Eagles, are unknown. The growing season in central–northern Quebec is approximately May–October, as based on monthly temperature data for the regions of Baie-James, Inukjuak, Kuujuak, Kuujuarapik, and Schefferville ( http://www.climat-quebec.qc.ca/home.php?id=norm_entab&mpn=stats), and nearly two-thirds of annual precipitation falls during this period. Furthermore, although we do not expect a 1:1 relationship between the time of precipitation and feather formation, the period of time that molt by Golden Eagles is thought to be most common is May–August. Thus, we developed geostatistical models and maps of May–August, May–October, and mean annual δ2Hp values for North America using the online workspace IsoMAP (Bowen et al. 2014). We used elevation, latitude, and latitude2 as independent variables in the models, since these factors strongly influence δ2H values of precipitation in North America (Bowen 2010). We did not include precipitation amount or longitude as independent variables in the models because they are not thought to have a large influence on spatial patterns of δ2Hp values in North America (Bowen 2010) and because they did not contribute significantly to our models in initial tests (data not shown). We extracted δ2Hp values from these isoscapes for the 8 adult Golden Eagles in our dataset with known summering grounds (7 individuals fitted with telemetry units, 1 museum specimen;  Supplementary Material Table S1). During initial analyses we found strong positive relationships between May–August and May–October δ2Hp values (r2 = 0.83), May–August and annual δ2Hp values (r2 = 0.99), and May–October and annual δ2Hp values (r2 = 0.88), and insignificant differences in the strength of the relationships between minimum δ2Hf values and May–August (r2 = 0.45), May–October (r2 = 0.46), and mean annual δ2Hp values (r2 = 0.47) for the 8 adult Golden Eagles with known summering grounds. The model and corresponding map for our May–August δ2Hp isoscape, which we chose to use for all additional analyses, are available as jobs 41698 and 41700 at  www.isomap.org, and the model summary is presented in  Supplementary Material Table S2. We also extracted δ2Hp values from the May–August isoscape for raptors of known origin (n = 264) for which δ2Hf data are available (Lott and Smith 2006), as described below.

We tested the ability of δ2Hf-based assignments to accurately record the summer grounds of eastern Golden Eagles of unknown origin using data from 8 adult birds with known summer grounds ( Supplemental Material Table S1). Because the feathers collected in the present study were obtained when the telemetry units were first deployed, the δ2Hf data provide information on where the bird was during the summer prior to capture (assuming that feathers form during the summer; see Discussion), whereas telemetry data provide location information after the time of capture. Therefore, we used adults because they establish distinct summer territories to which they return each year, whereas subadults exhibit less summer site fidelity within and between years (Miller 2012). For the 8 adults with known summer grounds, we used reduced major axis (RMA) regression to assess the relationships between their minimum δ2Hf values and (1) the latitude of their known summering grounds, and (2) the δ2Hp values of their summering grounds, as extracted from the May–August isoscape. We chose to use RMA regression because of symmetry between the dependent and independent variables (Smith 2009) and because both variables contain measurement uncertainty (McArdle 1988).

We also used RMA regression to determine the relationship between δ2Hf and May–August δ2Hp values for the 264 raptors of known origin in Lott and Smith (2006). The equation of the regression between δ2Hf and δ2Hp was: δ2Hf = 1.13(δ2Hp) + 11.15 (n = 264, r2 = 0.65, P < 0.001). To prepare the δ2Hf values of Golden Eagles for analysis in IsoMAP, they were rescaled to δ2Hp by rearranging the previous equation. We used the IsoMAP assignment tool, which is based on a Bayesian probabilistic framework, to map the likelihood-of-geographic-origin for feather δ2Hp values. These likelihood-of-origin maps are continuous probability surfaces. The standard deviation of δ2Hp values used for these assignments was set at 22‰, which is the standard deviation of the residuals of δ2Hp values in the previous equation relating δ2Hf and δ2Hp values for raptors of known origin (Lott and Smith 2006).

IsoMAP-based assignments span a longitudinal gradient across North America, but the summer nesting territories of eastern Golden Eagles are restricted to eastern Canada. Thus, we eliminated regions of biologically implausible origin by overlaying the approximate summer and year-round distribution of eastern Golden Eagles (Kochert et al. 2002, Wheeler 2007, Cadman et al. 2009, Katzner et al. 2012a, Morneau et al. 2015; Figure 1) on a likelihood-of-origin map of North America for each bird. We calculated the probability of origin (with contours set from 0.1 to 0.9 at intervals of 0.1) for each likelihood-of-origin map using the Geospatial Modelling Environment ( http://www.spatialecology.com/gme/), with each contour interval (“isopleth”) corresponding to a given percentage of pixels likely to contain the location of origin for an individual. For each of our 8 validation birds, we recorded the minimum isopleth contour that contained the centroid of the summering location. As a measure of the accuracy of our assignments, we then calculated the distances (direct, latitudinal, and longitudinal) between the centroid of the summering location and the centroid of the minimum isopleth contour that contained the summering location for all of the 8 validation birds. All pixels within this selected isopleth for each bird were reclassified as 1 (likely origin), and those outside as 0 (unlikely origin). We used the centroid of the likely region-of-origin for each bird as the isotope-inferred summering location of each bird. To visualize patterns of migratory connectivity, we stacked and summed each of the reclassified maps of likely summering regions for birds from each of 4 winter populations or groups (Figure 1). Statistical analyses were performed in PAST (Hammer et al. 2001).

RESULTS

Intra- and Interfeather Isotopic Variation

Average δ13C, δ15N, and δ2H values for the 42 Golden Eagles from which 2 or more feathers were obtained varied between −23.6 and −18.8‰, 4.7 and 13.2‰, and −161.4 and −25.7‰, respectively (Figure 2 and  Supplementary Material Figure S1). The average isotopic variation for δ13C and δ15N values between multiple feathers from the same bird (i.e. interfeather variation) was slightly larger than the average isotopic variation within feathers from the same bird (i.e. intrafeather variation; standard deviation = 0.52‰ and 0.60‰ vs. 0.39‰ and 0.44‰, respectively), whereas the average interfeather variation in δ2Hf values (standard deviation = 16.4‰) was more than double the average intrafeather variation in δ2Hf values (standard deviation = 7.9‰; Figure 2,  Supplementary Material Figures S1 and  S2). Interfeather variation in δ2H values was smaller for the juvenile birds (captured during their first winter) than for the older birds (Figure 2). For example, the average of the standard deviation of δ2Hf values among juveniles was 7.5‰, but it was 20.2‰ and 17.4‰ for subadults and adults, respectively. However, some of the older birds (e.g., 13D2700) displayed relatively little interfeather variation in δ2HfSupplementary Material Figure S2). The δ2H values of clips from near the proximal end of in-sheath feathers from birds captured on their wintering grounds were −34.2‰ and −40.3‰ for 2 birds from Alabama and −61.9‰ for a bird from northeastern West Virginia ( Supplementary Material Figure S2).

FIGURE 2.

Relationship between mean (A) δ15N and δ2H values, (B) δ13C and δ2H values, and (C) δ15N and δ13C values of feathers for Golden Eagles from which at least 2 feathers were analyzed and which were likely to have been eating terrestrial prey. Circles = juvenile birds, triangles = subadult birds, and squares = adult birds. Error bars represent the interfeather variation for each bird expressed as a standard deviation.

i0010-5422-117-3-414-f02.tif

Linking Isotope and Location Data to Assign Summer Locations

Northern latitudes generally have more negative δ2Hp values than do southern latitudes. Given this, and the relatively large intrabird variability in δ2Hf values for subadult and adult birds, we expected that the smallest δ2Hf value (as well as the corresponding δ2Hp value derived from the δ2Hf value) from each bird would most accurately record its most northerly (i.e. summer) location. We determined how well δ2H-based assignments recorded the summer grounds of 8 adult Golden Eagles with known breeding territories using the minimum δ2Hf value obtained from each bird. As expected, we observed a negative relationship between the minimum δ2Hf value from each of these adults and the latitude at which they summered (Figure 3A), as well as a positive relationship between the minimum δ2Hf value and the May–August δ2Hp value for the location at which they summered (Figure 3B). Furthermore, May–August δ2Hp values derived from the minimum δ2Hf values were within the 1σ range of variation of May–August δ2Hp values for 7 of the 8 birds (and just outside the 1σ range of variation for the remaining bird) for the locations at which these birds summered (Figure 3C). In addition, the mean slope and intercept of the relationship between δ2Hf and May–Aug δ2Hp of the raptor data in Lott and Smith (2006) [1.13 and 11.15, respectively, as stated above] are within the 95% confidence interval of the slope and intercept (0.0 to 3.1 and −91 to 191, respectively) of the relationship between δ2Hf and May–August δ2Hp for the 8 validation birds.

FIGURE 3.

Relationship between minimum δ2Hf (feather keratin) values and (A) summer latitude and (B) mean May–August δ2Hp (precipitation) values (derived from an isoscape of δ2Hp values) for 8 adult Golden Eagles with known summering grounds (latitude and longitude were determined from the centroid of the summering grounds). (C) Mean May–August δ2Hp values (triangles) and δ2Hp values calculated from minimum δ2Hf values (squares) using the relationship between δ2Hf and δ2Hp values for raptors for 8 adult Golden Eagles (arrayed along the x-axis) with known summering grounds. Error bars on May–August δ2Hp values represent one standard deviation.

i0010-5422-117-3-414-f03.tif

The minimum isopleth contour that contained the known summer centroid for each of the 8 validation birds ranged between 0.2 and 0.6. The locations of 5 of these 8 birds were within the 0.3 isopleth (data not shown). Thus, we drew a 0.6 isopleth on the likelihood-of-origin map for each Golden Eagle in our dataset (Figure 4). The average straight-line distance between the known centroid of the summering location for our 8 validation birds and the centroid of the 0.6 isopleth contour was 377 ± 263 km. The average north–south distance between the known centroid of the summering location for our 8 validation birds and the centroid of the 0.6 isopleth contour was 191 ± 129 km, whereas the average east–west distance was 307 ± 258 km.

FIGURE 4.

Likelihood-of-origin maps for 8 Golden Eagles with known summering locations. The stars represent the centroid of the summer location for each bird. The triangles represent the centroid of the 0.6 isopleth. The map area is the breeding range taken from Figure 1.

i0010-5422-117-3-414-f04.tif

Distribution and Migratory Connectivity

To identify the primary summer locations used by Golden Eagles, we used the minimum δ2Hf value from each bird to create a spatially explicit assignment of summering origin, as described above. The isotope-inferred summering locations of Golden Eagles varied between 52.1°N and 59.6°N. The greatest proportion of birds summered between 57°N and 58°N (Figure 5). As an initial test of potential leapfrog or chain migration by Golden Eagles in eastern North America, we assessed the relationship between wintering latitude and the minimum δ2Hf value from each bird. We found a positive relationship between these variables (Figure 6A), implying that birds that wintered farther north had more positive δ2Hf values (i.e. that they grew their feathers farther south). In addition, there was a negative relationship between wintering latitude and summering latitude (Figure 6B), also suggesting that birds that wintered farther north summered farther south. Finally, we found a negative relationship between wintering latitude and distance between the winter and summer grounds, implying that birds that wintered farther north migrated shorter distances. In fact, birds from the most southern wintering locations appeared to migrate approximately twice the distance as those from the most northern wintering locations (Figure 6C). These relationships are summarized in stacked maps (Figure 7), which indicate (1) the summering locations of birds from the 4 wintering groups, and (2) patterns of migratory connectivity, with greater percentages of birds from more northerly winter populations summering in more southerly areas of eastern Canada, and vice versa (Figure 7).

FIGURE 5.

Frequencies of summer latitude values for Golden Eagles inferred from likelihood-of-origin maps.

i0010-5422-117-3-414-f05.tif

FIGURE 6.

Relationships between (A) minimum δ2Hf values and wintering latitude, (B) the isotope-inferred summer latitude and wintering latitude, and (C) the distance migrated and wintering latitude for Golden Eagles in eastern North America. Note that the values on the x-axis of panel (A) are reversed.

i0010-5422-117-3-414-f06.tif

FIGURE 7.

Maps of the percentage of cells with a 1 (likely origin) for the summering grounds of Golden Eagles from each of the 4 winter populations (Figure 1). Birds from winter group 1 (n = 8) are shown in (A), birds from winter group 2 (n = 9) in (B), birds from winter group 3 (n = 14) in (C), and birds from winter group 4 (n = 7) in (D). The map extent is the known breeding range shown in Figure 1.

i0010-5422-117-3-414-f07.tif

DISCUSSION

Our results validate δ2H-based inferences of the summer locations of Golden Eagles of unknown origin. We showed that the majority of birds spent the breeding season in central Québec and northern Labrador. Further, our results indicated moderate patterns of leapfrog migration and migratory connectivity, with implications for the understanding of the evolution of migration, as well as the conservation, of Golden Eagles. Our use of telemetry tracking to confirm isotope-inferred movements is unique and helps to provide new insight into both the broad application of this technique and the specific distribution and migratory connectivity of Golden Eagles in eastern North America.

Validating Hydrogen Isotopes for Locating Summer Origins

We used a multistep process to evaluate the extent to which δ2Hf values may be useful for recording the geographic origin of eastern Golden Eagles. First, to constrain our analyses to terrestrial feeders, we identified feathers from birds with potential marine contributions to their diets (Lott et al. 2003). Only 2 of 47 birds had δ13C values >−20‰ and δ15N values >11‰ ( Supplementary Material Figure S1), which is the approximate cutoff for distinguishing apex predators, such as Golden Eagles, with terrestrial vs. mixed marine and terrestrial diets (e.g., Yerkes et al. 2008, Newsome et al. 2010). Thus, the majority of Golden Eagles in eastern North America eat primarily terrestrial prey during the period of feather molt. However, as a conservative measure, δ2Hf values from these 2 birds ( Supplementary Material Table S1) were excluded from subsequent analyses. Previous studies have suggested that birds are an important component of the diets of Golden Eagles in eastern North America during the summer (Spofford 1971, Brodeur and Morneau 1999). Our isotope data suggest that, to the extent that waterbirds are important, they are typically birds associated with terrestrial or freshwater, rather than marine, environments.

Second, to understand how within-individual processes may have affected the interpretation of our results, we assessed variation in δ13C, δ15N, and δ2H values within and among body feathers. We expected juvenile eagles to have the smallest inter- and intrafeather variation in isotopic values because the initial cohort of feathers on a Golden Eagle forms while the bird is in the nest (i.e. at a single location) during the summer. The relatively small interfeather variation in isotopic values for juvenile birds captured during the winter (Figure 2,  Supplementary Material Figures S1 and  S2) was consistent with this expectation. Juvenile birds do not begin molting new feathers, except adventitiously, until 8–12 mo following hatching. Thus, juvenile birds captured on their wintering grounds are unlikely to have molted many new feathers (Bloom and Clark 2001). In contrast, we expected older Golden Eagles to have greater inter- and intrafeather variation in isotopic values, since we expected their feathers to form at different times and places. The larger variation in isotopic values that we observed in the body feathers of older Golden Eagles was consistent with this expectation. The differences in interfeather variation in δ2Hf values among individuals suggested that the timing and location of molt differed among birds. Thus, δ2Hf data from juvenile birds captured during the winter are likely to reveal details about the location of the natal area, whereas δ2Hf data from subadults and adults will partly reveal the variation in locations where molting occurs. These results are consistent with those of prior studies, demonstrating that complex patterns of molt may be inferred from δ2Hf data (e.g., Perez and Hobson 2006). Overall, our results from body feathers agree with published observations that Golden Eagles replace flight and tail feathers throughout the year, with peak molting activity during mid-to-late summer (Bloom and Clark 2001). For migrant birds, such peak molting likely occurs when birds are at their northernmost locations, which have the most negative δ2Hp values.

Third, as a reference for winter δ2H values, we measured δ2H values from clips obtained near the proximal end of in-sheath feathers from 3 Golden Eagles captured on their wintering grounds ( Supplementary Material Figure S2). The portion of these feathers from which the clips were taken were unequivocally grown within the past week and therefore were formed on the wintering grounds (Edelstam 1984). All 3 samples had δ2Hf values >−62‰. Thus, we used this value as an approximate threshold to distinguish feathers formed in the eastern United States during winter (δ2Hf >−62‰) from feathers formed in eastern Canada during the summer (δ2Hf <−62‰).

Finally, we determined the accuracy of δ2H-based assignments for the 8 adult Golden Eagles for which we had sufficient information (telemetry data, summer capture data) to assess their summer locations. The negative relationship between minimum δ2Hf values and summer latitude and the positive relationship between the minimum δ2Hf values and the May–August δ2Hp values (Figures 3A and 3B) suggest that our approach of using the minimum δ2Hf value from each bird for geographic assignment using May–August δ2Hp values was appropriate. However, because of our relatively small sample size, this transfer function was unlikely to be robust for conversion of δ2Hf values to δ2Hp values for geospatial analysis. Thus, we followed the recommendation of Bowen et al. (2014) to evaluate whether a more generic transfer function based on our May–August δ2Hp isoscape was appropriate for use with Golden Eagles. To do so, we asked whether the relationship between δ2Hf values for the 264 raptors in the dataset of Lott and Smith (2006) and May–August δ2Hp values derived for those birds could be used to estimate May–August δ2Hp values from δ2Hf values of our 8 validation birds. We found (1) that May–August δ2Hp values derived from the minimum δ2Hf values of these 8 birds were within the range of variation of May–August δ2Hp values for the locations at which these birds summered (Figure 3C), and (2) that the mean slope and intercept of the relationship between δ2Hf and May–August δ2Hp values for the raptor data in Lott and Smith (2006) were within the 95% confidence interval of the slope and intercept of the relationship between δ2Hf and May–August δ2Hp values for the 8 validation birds. Thus, we conclude that it was appropriate to convert Golden Eagle δ2Hf values to May–August δ2Hp values (using the relationship that we derived from the Lott and Smith [2006] dataset) for geospatial analysis with a May–August δ2Hp isoscape.

Our results also illustrate the value of telemetry data for validating δ2H-inferred movements and migration pathways. This approach has received extensive use with marine animals (Pajuelo et al. 2012, Seminoff et al. 2012), but relatively little application to birds (Knoche et al. 2007, Bridge et al. 2014). Although isotope data provide less precise movement information than telemetry data, isotope data have important advantages. In particular, they are relatively inexpensive to collect and analyze, and they can be obtained from dead or injured individuals, individuals captured but not fitted with telemetry units, and individuals for which telemetry units fail. A coupling of telemetry and isotope data is likely to be invaluable for quantifying the summer distribution and patterns of migratory connectivity for other reclusive and difficult-to-track species.

Distribution and Migratory Connectivity

Our analyses illustrate the structure and degree of migratory connectivity of Golden Eagles that winter in the eastern United States. Nest survey data show that Golden Eagles have territories throughout eastern Canada (Morneau et al. 2015). However, identifying abundance from nest surveys is challenging because Golden Eagles occur at relatively low densities and the majority of the land area (>80%) in eastern Canada has not been surveyed for this species. Golden Eagles captured at any location during the winter in the eastern United States are most likely to have summered between 55°N and 58°N in eastern Canada (Figures 5 and 7). Presumably, large numbers of nesting sites and abundant prey make these latitudes quality habitat for Golden Eagles. Future study is required to determine whether Golden Eagles prefer these latitudes because of biotic factors (e.g., prey distributions), climatic factors (e.g., weather-driven determinants of lift), or other abiotic factors such as topography (e.g., nesting habitat or topographic determinants of lift).

Migratory connectivity has emerged as an important theme in avian ecology (Webster et al. 2002), with species demonstrating winter–summer linkages that range from strong (e.g., Delmore et al. 2012, Fort et al. 2012, Cormier et al. 2013) to weak (e.g., Renfrew et al. 2013, Trierweiler et al. 2014). Data from analysis of δ2Hf have provided evidence of chain migration by some species (e.g., Smith et al. 2003), leapfrog migration by others (e.g., Kelly et al. 2002, Clegg et al. 2003), and, in some cases, no clear evidence of leapfrog or chain migration (e.g., Symes and Woodborne 2010, Rushing et al. 2014). Invariant migration distances relative to the wintering or summering location is evidence for chain migration. Thus, the strong negative relationship that we observed between wintering latitude and distance between the wintering and summering grounds (Figure 6C) indicates that eastern Golden Eagles do not engage in chain migration. Furthermore, the positive relationship between wintering latitude and δ2Hf values (Figure 6A) and the negative relationship between wintering latitude and summering latitude (Figure 6B) suggest that these birds perform at least modest levels of leapfrog migration. Overall, there is a greater probability that migrants from the southern portion of the winter range originate from the northern portion of the summer range and vice versa. These results therefore confirm qualitative interpretations of migratory connectivity based on telemetry data from small numbers of Golden Eagles (Katzner et al. 2012b), and also illustrate how stable isotopes can provide a means of linking summer and winter distributions across the range of a migratory raptor.

The reasons for, and implications of, the observed patterns of migratory connectivity of eastern Golden Eagles are uncertain. For example, leapfrog migration could result from competition for the most favorable breeding or winter locations or from increased costs associated with longer migrations. If southern breeding locations are most favorable, then perhaps individuals may tolerate harsher winter conditions for the tradeoff of lesser energetic expenditures on migration (Berthold 1993), earlier arrival, and first choice of territories on breeding grounds. Regardless of their cause, these patterns of leapfrog migration are indicated in both behavior and the isotopic reflections of those behaviors, and they likely have important ecological and evolutionary implications. Documenting the summer and winter locations used by eastern Golden Eagles, as well as their patterns of migratory connectivity, thus provides a basis for better conservation, and increased understanding of the evolution of migration, of this species.

The modest degree of leapfrog migration that we observed implies that geographically variable threats could have differential impacts on subpopulations and long-term evolutionary potential. Although no Golden Eagle fatalities have yet to be reported at industrial-scale wind-energy facilities in the eastern United States, such fatalities are common in the western United States (Smallwood and Thelander 2008). Golden Eagles that summer in southern Canada are also those that winter primarily in the northeastern Appalachian Mountains, where there are increasing numbers of utility-scale wind-energy facilities. These birds could experience a disproportionately greater risk of wind-turbine mortality than those from the southwestern portion of the winter distribution where such facilities are uncommon. Overall, our results illustrate that leapfrog connectivity patterns present unique conservation challenges for Golden Eagles, including the fact that environmental changes may have spatially variable effects on population size and diversity.

ACKNOWLEDGMENTS

We thank numerous individuals, families, and institutions for assistance with fieldwork and data collection; David Brandes for providing feedback an earlier version of the manuscript; and Maria Wheeler for genetic analyses. We thank hundreds of bird banders who banded Golden Eagles, and Danny Bystrak from the Bird Banding Laboratory for providing Golden Eagle band encounter data. Two authors, T.K. and M.L., were, at the time of this research, owners of Cellular Tracking Technologies, the company that manufactured the GPS-GSM transmitters used in this project (T.K. has since divested). We thank MFFP (Ministry of Forests, Wildlife and Parks) for providing access to some of the data used in the manuscript. We thank Brian Millsap and two anonymous reviewers for comments that improved this paper. This is Scientific Article No. 3257 of the West Virginia Agricultural and Forestry Experiment Station, Morgantown.

Funding statement: Funding was provided by Pennsylvania State Wildlife Grants T-12 and T47-R-1, U.S. Department of Energy grant DE-EE0003538, the Charles A. and Anne Morrow Lindbergh Foundation, Hydro-Québec, the Virginia Department of Game and Inland Fisheries through a State Wildlife Grant from the U.S. Fish and Wildlife Service, and the authors' organizations. The funders did not have any influence on the content of the submitted manuscript nor did they require approval of the final manuscript.

Ethics statement: This work was conducted in accordance with appropriate Institutional Animal Care and Use (IACUC) protocols, including West Virginia University IACUC protocol #11-0304, and the Animal Care Committee of the Ministère des Forêts, de la Faune et des Parcs, Québec (permits CPA-FAUNE-07-00-02 and CPA-FAUNE-2009-06).

LITERATURE CITED

1.

T. Alerstam A. Hedenströmand S. Åkesson (2003). Long-distance migration: Evolution and determinants. Oikos 103:247–260. Google Scholar

2.

S. Bauerand B. J. Hoye (2014). Migratory animals couple biodiversity and ecosystem functioning worldwide. Science 344:1242552. doi: 10.1126/science.1242552 Google Scholar

3.

P. Berthold (1993). Bird Migration: A General Survey. Oxford University Press, New York, NY, USA. Google Scholar

4.

P. H. Bloomand W. S. Clark (2001). Molt and sequence of plumages of Golden Eagles and a technique for in-hand aging. North American Bird Bander 26:97–116. Google Scholar

5.

P. H. Bloom W. S. Clarkand J. W. Kidd (2007). Capture techniques. In Raptor Research and Management Techniques ( D. M. Birdand K. L. Bildstein Editors). Hancock House Publishers, Surrey, BC, Canada. pp. 193–220. Google Scholar

6.

C. Bothand M. E. Visser (2001). Adjustment to climate change is constrained by arrival date in a long-distance migrant bird. Nature 411:296–298. Google Scholar

7.

G. J. Bowen (2010). Isoscapes: Spatial pattern in isotopic biogeochemistry. Annual Review of Earth and Planetary Sciences 38:161–187. Google Scholar

8.

G. J. Bowen Z. Liu H. B. Vander Zanden L. Zhaoand G. Takahashi (2014). Geographic assignment with stable isotopes in IsoMAP. Methods in Ecology and Evolution 5:201–206. Google Scholar

9.

G. J. Bowen L. I. Wassenaarand K. A. Hobson (2005). Global application of stable hydrogen and oxygen isotopes to wildlife forensics. Oecologia 143:337–348. Google Scholar

10.

E. S. Bridge J. F. Kelly A. Contina R. M. Gabrielson R. B. MacCurdyand D. W. Winkler (2013). Advances in tracking small migratory birds: A technical review of light-level geolocation. Journal of Field Ornithology 84:121–137. Google Scholar

11.

E. S. Bridge J. F. Kelly X. Xiao J. Y. Takekawa N. J. Hill M. Yamage E. U. Haque M. A. Islam R. Mundkur K. E. Yavuz P. Leader et al . (2014). Bird migration and avian influenza: A comparison of hydrogen stable isotopes and satellite tracking methods. Ecological Indicators 45:266–273. Google Scholar

12.

S. Brodeurand F. Morneau (1999). Rapport sur la situation de l'aigle royal (Aquila chrysaetos) au Québec. Société de la faune et des parcs du Québec, Direction de la faune et des habitats, Québec, Canada. Google Scholar

13.

S. Brodeur R. Décarie D. M. Birdand M. Fuller (1996). Complete migration cycle of Golden Eagles breeding in northern Quebec. The Condor 98:293–299. Google Scholar

14.

M. Cadman D. Sutherlandand G. Beck (Editors) (2009). Atlas of the Breeding Birds of Ontario: 2001–2005. Bird Studies Canada, Toronto, ON, Canada. Google Scholar

15.

S. M. Clegg J. F. Kelly M. Kimuraand T. B. Smith (2003). Combining genetic markers and stable isotopes to reveal population connectivity and migration patterns in a Neotropical migrant, Wilson's Warbler (Wilsonia pusilla). Molecular Ecology 12:819–830. Google Scholar

16.

T. B. Coplenand H. P. Qi (2012). USGS42 and USGS43: Human-hair stable hydrogen and oxygen isotopic reference materials and analytical methods for forensic science and implications for published measurement results. Forensic Science International 214:135–141. Google Scholar

17.

R. L. Cormier D. L. Humple T. Gardaliand N. E. Seavy (2013). Light-level geolocators reveal strong migratory connectivity and within-winter movements for a coastal California Swainson's Thrush (Catharus ustulatus) population. The Auk 130:283–290. Google Scholar

18.

K. E. Delmore J. W. Foxand D. E. Irwin (2012). Dramatic intraspecific differences in migratory routes, stopover sites and wintering areas, revealed using light-level geolocators. Proceedings of the Royal Society of London, Series B 279:4582–4589. Google Scholar

19.

A. J. Dennhardt A. E. Duerr D. Brandesand T. E. Katzner (2015). Integrating citizen-science data with movement models to estimate the size of a migratory Golden Eagle population. Biological Conservation 184:68–78. Google Scholar

20.

C. De Ruyck K. A. Hobson N. Koper K. W. Larsonand L. I. Wassenaar (2013). An appraisal of the use of hydrogen-isotope methods to delineate origins of migratory Saw-whet Owls in North America. The Condor 115:366–374. Google Scholar

21.

C. Edelstam (1984). Patterns of moult in large birds of prey. Annales Zoologici Fennici 21:271–276. Google Scholar

22.

J. Fort E. Pettex Y. Tremblay S. H. Lorentsen S. Garthe S. Votier J. B. Pons F. Siorat R. W. Furness W. J. Grecian S. Bearhop et al . (2012). Meta-population evidence of oriented chain migration in Northern Gannets (Morus bassanus). Frontiers in Ecology and the Environment 10:237–242. Google Scholar

23.

A. K. Fridolfssonand H. Ellegren (1999). A simple and universal method for molecular sexing of non-ratite birds. Journal of Avian Biology 30:116–121. Google Scholar

24.

M. R. Fuller J. J. Millspaugh K. E. Churchand R. E. Kenward (2005). Wildlife radio telemetry. In Techniques for Wildlife Investigations and Management ( C. E. Braun Editor). The Wildlife Society, Bethesda, MD, USA. pp. 377–417. Google Scholar

25.

M. T. Hallworth C. E. Studds T. S. Sillettand P. P. Marra (2013). Do archival light-level geolocators and stable hydrogen isotopes provide comparable estimates of breeding-ground origin? The Auk 130:273–282. Google Scholar

26.

Ø. Hammer D. A. T. Harperand P. D. Ryan (2001). PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4:article 4.  http://palaeo-electronica.org/2001_1/past/issue1_01.htm Google Scholar

27.

X. A. Harrison D. J. Hodgson R. Inger K. Colhoun G. A. Gudmundsson G. McElwaine T. Tregenzaand S. Bearhop (2013). Environmental conditions during breeding modify the strength of mass-dependent carry-over effects in a migratory bird. PLOS One 8:e77783. doi: 10.1371/journal.pone.0077783 Google Scholar

28.

K. A. Hobson (1999). Tracing origins and migration of wildlife using stable isotopes: A review. Oecologia 120:314–326. Google Scholar

29.

K. A. Hobson (2011). Isotopic ornithology: A perspective. Journal of Ornithology 152:49–66. Google Scholar

30.

K. A. Hobson S. L. Van Wilgenburg J. Faaborg J. D. Toms C. Rengifo A. Llanes SosaY. Aubry, and R. Brito Aguilar(2014). Connecting breeding and wintering grounds of Neotropical migrant songbirds using stable hydrogen isotopes: A call for an isotopic atlas of migratory connectivity. Journal of Field Ornithology 85:237–257. Google Scholar

31.

K. A. Hobson S. L. Van Wilgenburg L. I. Wassenaarand K. Larson (2012). Linking hydrogen (δ2H) isotopes in feathers and precipitation: Sources of variance and consequences for assignment to isoscapes. PLOS One 7:e35137. doi: 10.1371/journal.pone.0035137 Google Scholar

32.

M. Jollie (1947). Plumage changes in the Golden Eagle. The Auk 64:549–576. Google Scholar

33.

T. E. Katzner D. Brandes T. Miller M. Lanzone C. Maisonneuve J. A. Tremblay R. Mulvihilland G. T. Merovich Jr (2012b). Topography drives migratory flight altitude of Golden Eagles: Implications for on-shore wind energy development. Journal of Applied Ecology 49:1178–1186. Google Scholar

34.

T. Katzner B. W. Smith T. A. Miller D. Brandes J. Cooper M. Lanzone D. Brauning C. Farmer S. Harding D. E. Kramar C. Koppie et al . (2012a). Status, biology, and conservation priorities for North America's eastern Golden Eagle (Aquila chrysaetos) population. The Auk 129:168–176. Google Scholar

35.

J. F. Kelly V. Atudorei Z. D. Sharpand D. M. Finch (2002). Insights into Wilson's Warbler migration from analyses of hydrogen stable-isotope ratios. Oecologia 130:216–221. Google Scholar

36.

J. F. Kelly E. S. Bridge A. M. Fudickarand L. I. Wassenaar (2009). A test of comparative equilibration for determining non-exchangeable stable hydrogen isotope values in complex organic materials. Rapid Communications in Mass Spectrometry 23:2316–2320. Google Scholar

37.

M. J. Knoche A. N. Powell L. T. Quakenbush M. J. Woollerand L. M. Phillips (2007). Further evidence for site fidelity to wing molt locations by King Eiders: Integrating stable isotope analyses and satellite telemetry. Waterbirds 30:52–57. Google Scholar

38.

M. N. Kochert K. Steenhof C. L. McIntyreand E. H. Craig (2002). Golden Eagle (Aquila chrysaetos). In The Birds of North America Online ( A. Poole Editor). Cornell Lab of Ornithology, Ithaca, NY, USA.  http://bna.birds.cornell.edu/bna/species/684 doi: 10.2173/bna.684 Google Scholar

39.

C. A. Lottand J. P. Smith (2006). A geographic-information-system approach to estimating the origin of migratory raptors in North America using stable hydrogen isotope ratios in feathers. The Auk 123:822–835. Google Scholar

40.

C. A. Lott T. D. Meehanand J. A. Heath (2003). Estimating the latitudinal origins of migratory birds using hydrogen and sulfur stable isotopes in feathers: Influence of marine prey base. Oecologia 134:505–510. Google Scholar

41.

B. H. McArdle (1988). The structural relationship—Regression in biology. Canadian Journal of Zoology 66:2329–2339. Google Scholar

42.

M. A. McCollough (1989). Molting sequence aging of Bald Eagles. Wilson Bulletin 101:1–10. Google Scholar

43.

T. Miller (2012). Movement ecology of Golden Eagles (Aquila chrysaetos) in eastern North America. Ph.D. dissertation, Pennsylvania State University, State College, PA, USA. Google Scholar

44.

T. A. Miller R. P. Brooks M. Lanzone D. Brandes J. Cooper K. O'Malley C. Maisonneuve J. Tremblay A. Duerrand T. Katzner (2014). Assessing risk to birds from industrial wind energy development via paired resource selection models. Conservation Biology 28:745–755. Google Scholar

45.

F. Morneau J. A. Tremblay C. Todd T. E. Chubbs C. Maisonneuve J. Lemaîtreand T. Katzner (2015). Known breeding distribution and abundance of Golden Eagle in eastern North America. Northeastern Naturalist 22:236–247. Google Scholar

46.

S. D. Newsome P. W. Collins T. C. Rick D. A. Guthrie J. M. Erlandsonand M. L. Fogel (2010). Pleistocene to historic shifts in Bald Eagle diets on the Channel Islands, California. Proceedings of the National Academy of Sciences USA 107:9246–9251. Google Scholar

47.

R. M. Nielson L. McManus T. Rintz L. L. McDonald R. K. Murphy W. H. Howeand R. E. Good (2014). Monitoring abundance of Golden Eagles in the western United States. Journal of Wildlife Management 78:721–730. Google Scholar

48.

D. R. Norris P. P. Marra T. K. Kyser T. W. Sherryand L. M. Ratcliffe (2004). Tropical winter habitat limits reproductive success on the temperate breeding grounds in a migratory bird. Proceedings of the Royal Society of London, Series B 271:59–64. Google Scholar

49.

M. Pajuelo K. A. Bjorndal K. J. Reich M. D. Arendtand A. B. Bolten (2012). Distribution of foraging habitats of male loggerhead turtles (Caretta caretta) as revealed by stable isotopes and satellite telemetry. Marine Biology 159:1255–1267. Google Scholar

50.

K. L. Paxton C. Van Riper III T. C. Theimerand E. H. Paxton (2007). Spatial and temporal migration patterns of Wilson's Warbler (Wilsonia pusilla) in the Southwest as revealed by stable isotopes. The Auk 124:162–175. Google Scholar

51.

G. E. Perezand K. A. Hobson (2006). Isotopic evaluation of interrupted molt in northern breeding populations of the Loggerhead Shrike. The Condor 108:877–886. Google Scholar

52.

P. Pyle (1997). Identification Guide to North American Birds, Part I: Columbidae to Ploceidae. Slate Creek Press, Point Reyes Station, CA, USA. Google Scholar

53.

R. B. Renfrew D. Kim N. Perlut J. Smith J. Foxand P. P. Marra (2013). Phenological matching across hemispheres in a long-distance migratory bird. Diversity and Distributions 19:1008–1019. Google Scholar

54.

S. Rohwer R. E. Ricklefs V. G. Rohwerand M. M. Copple (2009). Allometry of the duration of flight feather molt in birds. PLOS Biology 7:e1000132. doi: 10.1371/journal.pbio.1000132 Google Scholar

55.

C. S. Rushing T. B. Ryder J. F. Saraccoand P. P. Marra (2014). Assessing migratory connectivity for a long-distance migratory bird using multiple intrinsic markers. Ecological Applications 24:445–456. Google Scholar

56.

J. A. Seminoff S. R. Benson K. E. Arthur T. Eguchi P. H. Dutton R. F. Tapilatuand B. N. Popp (2012). Stable isotope tracking of endangered sea turtles: Validation with satellite telemetry and δ15N analysis of amino acids. PLOS One 7:e37403. doi: 10.1371/journal.pone.0037403 Google Scholar

57.

K. S. Smallwoodand C. Thelander (2008). Bird mortality in the Altamont Pass Wind Resource Area, California. Journal of Wildlife Management 72:215–223. Google Scholar

58.

A. D. Smithand A. M. Dufty (2005). Variation in the stable-hydrogen isotope composition of Northern Goshawk feathers: Relevance to the study of migratory origins. The Condor 107:547–558. Google Scholar

59.

R. B. Smith T. D. Meehanand B. O. Wolf (2003). Assessing migration patterns of Sharp-shinned Hawks Accipiter striatus using stable-isotope and band encounter analysis. Journal of Avian Biology 34:387–392. Google Scholar

60.

R. J. Smith (2009). Use and misuse of the reduced major axis for line-fitting. American Journal of Physical Anthropology 140:476–486. Google Scholar

61.

W. R. Spofford (1971). The breeding status of the Golden Eagle in the Appalachians. American Birds 25:3–7. Google Scholar

62.

C. Q. Stanley E. A. McKinnon K. C. Fraser M. P. Macpherson G. Casbourn L. Friesen P. P. Marra C. Studds T. B. Ryder N. E. Diggsand B. J. M. Stutchbury (2015). Connectivity of Wood Thrush breeding, wintering, and migration sites based on range-wide tracking. Conservation Biology 29:164–174. Google Scholar

63.

C. T. Symesand S. Woodborne (2010). Migratory connectivity and conservation of the Amur Falcon Falco amurensis: A stable isotope perspective. Bird Conservation International 20:134–148. Google Scholar

64.

R. E. Tingayand T. E. Katzner (Editors) (2010). The Eagle Watchers. Cornell University Press, Ithaca, NY, USA. Google Scholar

65.

C. Trierweiler R. H. G. Klaassen R. H. Drent K.-M. Exo J. Komdeur F. Bairleinand B. J. Koks (2014). Migratory connectivity and population-specific migration routes in a long-distance migratory bird. Proceedings of the Royal Society of London, Series B 281:20132897. doi: 10.1098/rspb.2013.2897 Google Scholar

66.

J. G. B. van Dijk W. Meissnerand M. Klaassen (2014). Improving provenance studies in migratory birds when using feather hydrogen stable isotopes. Journal of Avian Biology 45:103–108. Google Scholar

67.

L. I. Wassenaar (2008). An introduction to light stable isotopes for use in terrestrial animal migration studies. In Tracking Animal Migration with Stable Isotopes ( K. A. Hobsonand L. I. Wassenaar Editors). Academic Press, London, England. pp. 21–44. Google Scholar

68.

L. I. Wassenaarand K. A. Hobson (2003). Comparative equilibration and online technique for determination of non-exchangeable hydrogen of keratins for use in animal migration studies. Isotopes in Environmental and Health Studies 39:211–217. Google Scholar

69.

L. I. Wassenaarand K. A. Hobson (2006). Stable-hydrogen isotope heterogeneity in keratinous materials: Mass spectrometry and migratory wildlife tissue subsampling strategies. Rapid Communications in Mass Spectrometry 20:2505–2510. Google Scholar

70.

J. Watson (2010). The Golden Eagle. Yale University Press, New Haven, CT, USA. Google Scholar

71.

M. S. Webster P. P. Marra S. M. Haig S. Benschand R. T. Holmes (2002). Links between worlds: Unraveling migratory connectivity. Trends in Ecology & Evolution 17:76–83. Google Scholar

72.

B. K. Wheeler (2007). Raptors of Eastern North America: The Wheeler Guides. Princeton University Press, Princeton, NJ, USA. Google Scholar

73.

A. E. Wiley A. J. Welch P. H. Ostrom H. F. James C. A. Stricker R. C. Fleischer H. Gandhi J. Adams D. G. Ainley F. Duvall N. Holmes et al . (2012). Foraging segregation and genetic divergence between geographically proximate colonies of a highly mobile seabird. Oecologia 168:119–130. Google Scholar

74.

T. Yerkes K. A. Hobson L. I. Wassenaar R. Macleodand J. M. Coluccy (2008). Stable isotopes (δD, δ13C, δ15N) reveal associations among geographic location and condition of Alaskan Northern Pintails. Journal of Wildlife Management 72:715–725. Google Scholar
© 2015 Cooper Ornithological Society.
David M. Nelson, Melissa Braham, Tricia A. Miller, Adam E. Duerr, Jeff Cooper, Michael Lanzone, Jérôme Lemaître, and Todd Katzner "Stable hydrogen isotopes identify leapfrog migration, degree of connectivity, and summer distribution of Golden Eagles in eastern North America," The Condor 117(3), 414-429, (12 August 2015). https://doi.org/10.1650/CONDOR-14-209.1
Received: 9 December 2014; Accepted: 1 June 2015; Published: 12 August 2015
JOURNAL ARTICLE
16 PAGES


SHARE
ARTICLE IMPACT
Back to Top