The venoniine wolf spider genus Allotrochosina Roewer 1960 currently includes two species, A. schauinslandi Simon 1899 from New Zealand (type species) and A. karri Vink 2001 from Western Australia. A third species of this genus, A. walesiana new species from New South Wales, Australia, is here described. Some differences in genital morphology of A. walesiana in comparison to A. schauinslandi and A. karri in combination with a misinterpretation of genitalic characters in previous treatments of the genus require a new diagnosis for Allotrochosina. The genus is characterized by the presence of a distinct apical process on the embolic division of the male pedipalp. Allotrochosina walesiana appears to be winter mature since adult spiders have only been found between June and October. Additional records of A. karri, which was previously only known from the type locality, Crowea in southwest Western Australia, extend the known distribution by more than 600 km to the North.