The abundance and diversity of gymnamoebae in three subsoils varying in compaction and water retention along a 1.2 m transect were documented as the local climatic conditions changed from late summer 1999 through mid-summer 2000. The mean density of gymnamoebae for the loose soil (1,655/g) was greater than either the most compact (1,468/g) or moderately compact soil (851/g). Minimum densities occurred in middle and late summer for all soils while significant (F = 38.803, ≤ 0.0002) density peaks at 3,212/g occurred in early summer in the most compact soil, 2,928/g in the least compact, and 2,209/g in the moderately compact soil. Limax non-eruptive gymnamoebae (mt 2) correlated (r = 0.49, p ≤ 0.016) with moisture while eruptive limax gymnamoebae (mt 3) correlated with temperature (r = 0.07, p ≤ 0.024), moisture (r = 0.58, p ≤ 0.001) and precipitation (r = 0.46, p ≤ 0.029). Flattened or discoid amoebae (mt 4) dominated throughout most of the survey, and the two limax groups showed inverse relationships. Chi-square analyses showed significant differences in the numbers of limax eruptive gymnamoebae compared to all other morphotypes on all but one sampling period.