The New Jersey Pine Barrens (Pinelands) is the primary resource for the identification of many of the Zygnematophyceae described in the United States by Francis Wolle and published in early volumes of the Bulletin of the Torrey Botanical Club. Based on the current study, more than 1,100 algal species and infraspecific taxa exist in the Pinelands, which is more than all the other algal studies for the Pinelands combined. This report lists 545 Zygnematophyceae taxa plus 15 genus species. From this list, there are 316 taxa newly reported from 90 current sites within 17 New Jersey Pinelands watersheds.