We studied spatial differences in Barn Owl (Tyto alba stertens) diets in agroecosystems of six districts of central Punjab, Pakistan. Analysis of pellets collected over 3 years revealed the house shrew (Suncus murinus) dominated all diets. This species constituted 75.0% of the diet in the Sheikhupura District, 68.4% in the Okara District, 67.2% in the Faisalabad District, 65.6% in the Toba Tek Singh District, 59.3% in the Jhang District, and 56.3% in the Hafizabad District. Rats and mice together formed 28% of the overall diet while birds (4.2%) were consumed more than bats (2.0%). The greatest diversity in Barn Owl diets was in the Jhang District.