The avian genus Chrysothlypis has 2 species distributed in Central and South America. We report the first description of the nest and breeding behavior of the Scarlet-and-white Tanager (Chrysothlypis salmoni) from the Colombian Chocó. A single nest was found and monitored in March 2018 during a field season in the Anchicayá Valley, Valle del Cauca, Colombia. The nest resembles the nest of congeners in the subfamily Hemithraupinae: a cup constructed with rootlets, leaves, fungal rhizomorphs, moss, and spiderwebs. It was placed 6 m above ground in a Cespedecia spathulata tree located at the forest edge alongside a gravel road. We observed only the female brooding a single chick, but the male helped feed the nestling. Groups of vocal individuals visited the nest and, on one occasion, a suspected helper fed the nestling. Our study presents the first nesting information for this species.