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1 September 2008 A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for Scanning Electron Microscope
María M. Ronderos, Florentina Díaz, Patricia Sarmiento
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Abstract

A new procedure for clearing eggs belonging to genera of Ceratopogonidae is described. In the new procedure eggs in different states, with different ultra structures of the exochorion, were immersed in 10% glycolic acid (CH2OH-COOH) and 20 % mandellic acid (C6H5.CHOH.COOH) for 1–4 minutes. Eggs, prepared by both acids and subsequently neutralized by distilled water, were placed in a saturated solution of phenol, and then passed through a battery of alcohol solutions. Finally, critical point was performed and gold metallized. After preparation the eggs of this group retained their natural form under vacuum long enough to yield good microphotographs of the outer layer of the chorion.

María M. Ronderos, Florentina Díaz, and Patricia Sarmiento "A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for Scanning Electron Microscope," Transactions of the American Entomological Society 134(3), 471-476, (1 September 2008). https://doi.org/10.3157/0002-8320-134.3.471
Published: 1 September 2008
JOURNAL ARTICLE
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