The scope of this study is to evaluate the faunistic, ecological and geographical composition of the subfamily Aleocharinae (Coleoptera: Staphylinidae) of the Eastern Black Sea Region of Turkey. Eighty species were detected by field studies between 2013 and 2015 in the Eastern Black Sea Region. We concluded that the following five species – Aleochara binotata Kraatz, 1856, A. ruficornis Gravenhorst, 1802, Atheta liturata Stephens, 1832, Gyrophaena poweri Crotch, 1867, Zyras collaris (Paykull, 1800) – are new records for Turkey. Seven species are endemic for Turkey and 40 species are new records for the Eastern Black Sea Region. Also, detailed locality records of two species, Aleochara moerens Gyllenhal, 1827 and Atheta oblita (Erichson, 1839), are given for Turkey.