Road kills impact many wildlife species, but only a limited number of studies have included small animals during these surveys. This study described the road mortality for large, medium and small vertebrates, which includes amphibians, reptiles, small mammals and birds. A 32.2 km stretch of Kansas Highway K254 was surveyed from 31 May 1985 thru 20 May 1987. Eighty-eight species comprised the 4484 individuals found during this study. The road mortality for mammal and bird groups here resembled those reported in other studies, but differed from those reported for herpetofauna group. Ornate box turtle, prairie kingsnake and gopher snake sustained higher road mortalities than found in previous studies. Wildlife also experienced greater road mortality in a landscape where prairie occurred in large contiguous tracts compared with a patchwork of small stands. This study showed that surveying a limited stretch of highway represents a feasible way to obtain accurate road mortality data.