Background and Research: One noteworthy element found throughout the tropical anthropogenic mosaic is the live fence, which is established within agricultural matrices and its structure within the landscape retains ecological processes, but few are recognized as elements of biological and cultural conservation.

Methods: In this study, we have researched plant diversity and anthropic management of live fences in five sites surrounded by contrasting vegetation references: Tropical evergreen forest; tropical deciduous forest; cloud forest; and pine–oak and pine forests. We recorded the type of management by interviews with peasants. We established thirty 2 × 50 m transects within each site and sampled two strata: trees and saplings. Also, we documented seed dispersal mechanism, life form, local use, and origin of each species. Importance Value Index and diversity metrics were estimated for each site.

Results: 253 plant species were registered (181 genera/74 families). While fences associated with the tropical deciduous forest showed the greatest species richness (109 species), the pine forest fences showed the lowest richness (21 species). Zoochory was the main type of seed dispersal mechanism.

Conclusions: Independent to the site and the altitude, the configuration of living fences is structured by three processes: the selection of the initial trees, the availability of the arrival of zoochory species, and the tolerance of the owners for the plant species.

Implications for Conservation: Based on our results, live fences can be considered important tools for landscape management in Mexico.