Native bee community diversity is known to vary temporally in the Neotropics, but little research has been done to quantify the extent of this variation. We studied monthly variation in native bee diversity in tropical semi-deciduous forest on the Yucatan Peninsula, Mexico, over a twelve-month period in 2007. Hurricane Dean traversed the study area in August of that year, causing some changes in the environment and in the native bee community. A total of 2,335 individual bee specimens were collected, belonging to 102 species from four families. The family Apidae had the highest number of species (46%) and individuals (71%). Species richness, abundance, and diversity varied throughout the study period. Species proportions of lifestyles and nesting behaviors varied from month to month, and absolute numbers changed. Low to moderate similarity values indicated high monthly species turnover, with almost half of species present for only one to two months. Species richness declined after the hurricane, with a loss of 40% of the species present beforehand, but nine new species appeared in September. The hurricane also reduced the number of solitary, parasocial, and social species, mostly those nesting in preexisting cavities and wood. Species composition in August had similarity values nearer to those of months in the dry season than to those in the rainy season. Two months after the hurricane, the native bee community had returned to previous species diversity levels, probably due to the rapid recovery of the vegetation on which the bee community depends. Effective biodiversity conservation programs will need more data on changes in vegetation community structure and how such changes influence Neotropical native bee communities after natural disturbances.