Goosegrass [Eleusine indica (L.) Gaertn.] is one of the most problematic grassy weeds in the world. It is considered to be an important weed in summer fallows and crops grown in the eastern region of Australia. To examine the seed germination ecology of two populations (Gatton and Ingham) of E. indica and their response to postemergence herbicides in Australian conditions, experiments were carried out in the laboratory and screenhouse. Seedling survival, spike production, and plant biomass of both E. indica populations declined markedly with the application of postemergence herbicides such as butroxydim, clethodim, glufosinate, haloxyfop, and propaquizafop, whereas the application of paraquat failed to control the Ingham population. A dose–response study verified the presence of paraquat resistance in the Ingham population. In this regard, it was observed that the paraquat doses required to achieve a 50% reduction in survival and plant biomass were 27 and 21 times greater in the Ingham population compared to the Gatton population, respectively. Higher alternating temperatures (35/25 and 30/20 C) resulted in greater germination of both populations than lower alternating temperatures (20/10 and 25/15 C). At 20/10 C, the Ingham population failed to germinate; however, about 15% germination in the Gatton population was observed. At the lowest alternate temperature range (15/5 C), neither population germinated. The germination of both populations of E. indica was severely reduced under completely dark conditions compared with the alternating light/dark period. Germination was more tolerant of salt and water stress in the Ingham population compared with the Gatton population. Eleusine indica seedling emergence was comparable among populations, and the greatest emergence (83%) was observed for seeds buried at a depth of 2 cm but then declined dramatically, and no seedlings emerged from an 8-cm burial depth. The information acquired from this study could be used in developing effective management strategies for E. indica.