The eastern woodrat (Neotoma floridana) occurs in eastern and central parts of the United States, with 3 subspecies inhabiting Nebraska. Herein, we report on distributional records of N. f. baileyi—an isolated, disjunct subspecies in north-central Nebraska. We searched for diagnostic sign of woodrats in 4 counties in Nebraska and 1 county in South Dakota. We obtained distributional records for N. f. baileyi east, west, and north of known distribution limits in Nebraska, but none in South Dakota. Most distributional records represent range extensions because of lack of prior mammalian surveys in those areas. Observations of woodrats in isolated, human-established woodlots surrounded by prairies represent range expansions because such habitats were not present in the past.