Interspecies interactions can affect how species are distributed, put constraints on habitat expansion, and reduce the fundamental niche of the affected species. Using logistic regression, we analyzed and compared 174 Tamias palmeri and 94 Tamias panamintinus within an isolated mountain range of the Basin and Range Province of southern Nevada. Tamias panamintinus was more likely to use pinyon/ponderosa/fir mixed forests than pinyon alone, compared to random sites. In the presence of T palmeri, however, interaction analyses indicated T. panamintinus was less likely to occupy the mixed forests and more likely near large rocks on southern aspects. This specie s-by-habitat interaction data suggest that T. palmeri excludes T panamintinus from areas of potentially suitable habitat. Climate change may adversely affect species of restricted distribution. Habitat isolation and species interactions in this region may thus increase survival risks as climate temperatures rise.