The Sutter Buttes (Buttes) are a small, isolated mountain range in the Great Central Valley of California. Systematic study of the fauna and flora of this unique habitat has been limited by the private ownership of the entire mountain range. As a result, much of our knowledge about the herpetofauna of the Buttes is based on isolated observations and scattered museum records, most over 4 decades old. In this study, we present an updated inventory of the herpetofauna of the Buttes, based on a 2-year field survey within the boundaries of the first publicly protected property within the Buttes (Sutter Buttes State Park) and a comprehensive survey of museum specimens. The objective of this study was to develop a current herpetofaunal species list for the Buttes as a whole and the park specifically. We verify the occurrence of 16 previously identified herpetofaunal species within the park and provide evidence for 3 additional species. We also provide the first record of the widely invasive Pond Slider (Trachemys scripta); clarify the status of a previous misidentification of a federally threatened species, Giant Gartersnake (Thamnophis gigas); and provide evidence for the probable extirpation of 2 California Species of Special Concern, the Foothill Yellow-Legged Frog (Rana boylii) and Blainville's Horned Lizard (Phrynosoma blainvillii). Therefore, our species list for the Buttes includes 20 herpetofaunal species. Museum specimens from the Buttes are located at 8 museums, with the majority at the California State University, Chico Vertebrate Museum. Sixteen of the 22 herpetofauna species extant or recently extirpated from the Buttes are represented by museum vouchers, with only 11 having tissue samples. This study sets an updated baseline for the further study and monitoring that will be required to facilitate effective management and conservation of the unique Sutter Buttes.