In the present paper, three new species of Lecteria (Lecteria) Osten Sacken,1888: Lecteria (Lecteria) mitarakanea n. sp., Lecteria (Lecteria) teko n. sp. and Lecteria (Lecteria) wayana n. sp., are described and illustrated, as a first step in the study of the Tipulidae Latreille, 1802 fauna from Mitaraka (French Guiana), collected during the “Our Planet Revisited” survey of 2015. The characteristics of the male genitalia allow to easily separate these three new species from the rest of the Neotropical species, in combination with the color pattern of legs, thorax and wings. The species are the first described in this genus from the Neotropics in almost half a century. Their discovery illustrates the significant contribution of this kind of scientific surveys in remote and isolated spots around the globe.