The aim of this study was to evaluate the effect of the enzymatic extract (EnzE) of native fungus Trametes maxima CU1 on the productive parameters and carcass yield of rabbits. A total of 36 rabbits, 18 New Zealand and 18 California breeds were distributed randomly into two treatments: control (without EnzE supplementation) and EnzE2.5 (with 2.5% of enzymatic extract added to drinking water). All rabbits were fed with a commercial diet ad libitum. At 49, 71, and 91 d, data for body weight (BW), average daily feed intake (ADFI), feed efficiency, and average daily gain were collected. Moreover, dressing out percentage (DoP) and carcass fat yield (�Y) were estimated. BW and ADFI were not different between treatments (P > 0.05). However, rabbits supplemented with EnzE2.5 showed higher values than the control. Rabbits EnzE2.5 and New Zealand males showed the best productive efficiency at 49 d (P < 0.05). On the other hand, EnzE2.5 showed greater DoP than control; furthermore, EnzE2.5 did not show any effect over �Y. These results show the potential of T. maxima CU1 as a source of lignocellulases and amylases for the improvement of productive behavior and carcasses yield.