Asplenium serratifolium (Aspleniaceae), a new fern species from central Vietnam, is described and illustrated. The new species is characterized by plants 10–18 cm tall, laminae pinnatipartite, lobe margins entire or with shallow teeth, and veins simple or forked. Molecular phylogenetic analysis based on five plastid markers (atpB, rbcL, rps4, rps4-trnS, and trnL-F) indicate that the new species is closely related to A. ensiforme.