The effects of somatostatin (SRIF) on in vitro avian adrenal steroid secretion have been investigated in the domestic turkey (Meleagris gallopavo) and fowl (Gallus gallus domesticus). SRIF did not affect either basal or ACTH-stimulated aldosterone and corticosterone production by dispersed turkey adrenocortical cells, but it concentration-dependently inhibited the secretory response to angiotensin II (ANG II). It is concluded that in the turkey, like in mammals, SRIF specifically interferes with the intracellular mechanisms transducing the ANG II secretagogue signal. Fowl adrenocortical cells did not display any secretory response to ANG II, and accordingly SRIF did not alter their basal and agonist-stimulated secretory activity.
Many lines of evidence indicate that somatostatin (SRIF) plays an important physiological role in the modulation of adrenal mineralocorticoid secretion in mammals (for review, see Nussdorfer, 1996). However, the investigations dealing with the effect of SRIF on adrenal steroid secretion in lower vertebrates are very scarce and their findings are rather conflicting. Delarue et al. (1984) and Feuilloley et al. (1994) did not observe any effect of SRIF and urotensin II (a cyclic dodecapeptide exhibiting structural similarities with mammalian SRIF) on either basal or angiotensin II (ANG II)- stimulated aldosterone secretion by perifused frog adrenals. In contrast, Hanke and Kloas (1994) reported that urotensin II enhances adrenal steroid release in Amphibia. Studies on this matter are almost completely lacking in Aves: at present, only one investigation is available providing indirect evidence that SRIF may be involved in the negative regulation of adrenal steroid secretion in the rooster (Cheung et al., 1988).
It, therefore, seemed worthwhile to examine the effect of SRIF on in vitro steroid secretion of adrenocortical cells of two avian species, the domestic fowl and turkey.
MATERIALS AND METHODS
Male domestic fowls (Gallus gallus domesticus) and turkeys (Meleagris gallopavo) of three months of age were obtained from the breeding facilities of our Agriculture School. The animals were decapitated, and their adrenal glands promptly removed. Glands were minced and stored in ice-cold Krebs-Ringer bicarbonate buffer with 0.2% glucose (KRBG), and dispersed cells were obtained by sequential collagenase digestion and mechanical disaggregation (Szalay, 1981). Dispersed cells were then collected and adrenocortical cells were separated from chromaffin cells on a preformed continuous density Percoll gradient, as described by Kocsis et al. (1994a). Dispersed cells obtained from adrenals of 2 animals were pooled to obtain a single cell suspension, and 6 cell preparations for each incubation experiment were employed.
Dispersed adrenocortical cells were put in a 2:1 mixture of Medium 199 (DIFCO, Detroit, MI) and KRBG, containing 5 mg/ml bovine serum albumin, and incubated (3 × 105 cells/ml) with the following peptides purchased by Sigma Chemical Co. (St. Louis, MO). (i) SRIF (from 10−10 to 10−5 M) in the presence or absence of 10−9 M ACTH or ANG II; and (ii) 10−9 M ANG II and 10−6 M cyclo(7-aminoheptanoyl-Phe-D- Trp-Lys-Thr[Bzl]) (SRIF-A), a specific competitive antagonist of SRIF receptors (Fries et al., 1982), in the presence or absence of 10−7 M SRIF. The incubation was carried out in a shaking bath at 37°C for 90 min, in an atmosphere of 95% O2 −5% CO2.
Aldosterone and corticosterone concentrations in the incubation media were measured, after extration and HPLC purification (Neri et al., 1993), by specific RIA, as described previously (Malendowicz et al., 1993). Intra- and interassay variations were: aldosterone, 7% and 8%; corticosterone, 6% and 9%, respectively.
Data were averaged per experimental group and SEM (n = 6) were calculated. Their statistical comparison was done by ANOVA followed by the Multiple Range Test of Duncan.
SRIF did not alter either basal or 10−9 M ACTH-stimulated aldosterone (Fig. 1) and corticosterone (Fig. 2) production by turkey interrenal cells. In contrast, it concentration-dependently inhibited ANG II (10−9 M)-enhanced hormonal secretion; minimal and maximal effective concentrations (10−8 M and 10−7 M, respectively) caused about 20% and 40% inhibitions (Figs. 1 and 2). The suppression of aldosterone and corticosterone responses to 10−9 M ANG II evoked by 10−7 M SRIF was abrogated by the simultaneous exposure to 10−6 M SRIF-A, which per se did not affect basal steroid secretion (Table 1).
Effect of 10−6 M SRIF-A on SRIF (10−7 M)-induced inhibition of secretory response of dispersed turkey adrenocortical cells to 10−9 M ANG II
SRIF was not found to induce significant changes in either basal or agonist-stimulated steroid secretion of dispersed fowl interrenal cells, which responded to ACTH, but not to ANG II (Figs. 1 and 2).
Our present findings clearly indicate that SRIF, acting via specific receptors, directly inhibits the steroidogenic response of turkey adrenocortical cells to ANG II. A large body of evidence indicates that in mammals SRIF specifically depresses secretion and growth of adrenal zona glomerulosa cells, by interfering with the main signaling mechanism transducing the ANG II secretagogue signal (i.e. the intracellular cascade following phospholipase C activation) (for review, see Nussdorfer, 1996). Hence, it may be conceived that the same mechanism underlies the inhibitory action of SRIF in the turkey, inasmuch as this peptide does not alter the secretory response of adrenocortical cells to ACTH, which is known to act by activating adenylate cyclase (for review, see Ganguly and Davis, 1994). However, it must be recalled that maximal effective concentrations of SRIF cause only a 40% inhibition of the secretory response to ANG II. This observation may be explained by taking into account that the mechanism underlying the secretagogue action of ANG II involves the activation of both phosphoinositide and tyrosine kinase pathways (Bodart et al., 1995; Kapas et al., 1995). Preliminary data (not shown), indicating that the selective tyrosine kinase inhibitor tyrphostin 23 (but not the phosphokinase C antagonist Ro31-8220) potentiates the effect of SRIF, strongly suggest that this peptide exclusively acts by blocking ANG II-stimulated phosphoinositide pathway in the turkey adrenocortical cells.
According to previous studies (Rosenberg et al., 1988; Holmes and Cronshaw, 1993), dispersed fowl adrenocortical cells, though displaying a clear-cut secretory response to ACTH, are insensitive to ANG II. Probably, adrenocortical cells of this species, at variance with those of the turkey (Kocsis et al., 1994b), are not provided with specific ANG II receptors; alternatively they could possess that subclass of ANG II receptors not coupled with a secretory response, which has been recently demonstrated to occur in the turkey adrenocortical cells (Kocsis et al., 1995). Be that as it may, these findings easily explain why SRIF does not exert any direct inhibitory action on the secretion of fowl adrenocortical cells.
Cheung et al. (1988) have been reported that the in vivo administration of an anti-SRIF serum raises the basal blood level of corticosterone in the domestic chicken, thereby suggesting that endogenous SRIF plays a role in the regulation of adrenal steroidogenesis. Our present findings appear to conflict with this contention. A clue to reconcile this apparent discrepancy could be provided by the investigations showing that in mammals SRIF may regulate adrenal cortex indirectly by inhibiting the hypothalamo-pituitary CRH-ACTH system (Richardson and Schonbrunn, 1981; Reisine, 1985), as well as the release by adrenomedullary chromaffin cells of catecholamines (Moeller et al., 1989), which are known to enhance basal adrenal steroid secretion (for review, see Nussdorfer, 1996). It remain to be settled whether these indirect mechanisms of action of SRIF are operative also in the Aves.
- V. Bodart, H. Ong, and A. De Léan . 1995. A role for protein tyrosine kinase in the steroidogenic pathway of angiotensin II in bovine zona glomerulosa cells. J Steroid Biochem Mol Biol 54:55–62. Google Scholar
- A. Cheung, S. Harvey, T. R. Hall, S. K. Lam, and G. S. G. Spencer . 1988. Effects of passive immunization with antisomatostatin serum on plasma corticosterone concentrations in young domestic cockerels. J Endocrinol 116:179–183. Google Scholar
- C. Delarue, P. Netchitailo, F. Leboulenger, I. Perroteau, E. Escher, and H. Vaudry . 1984. In vitro study of frog (Rana ridibunda Pallas) interrenal function by use of a simplified perifusion system. VII. Lack of effect of somatostatin on angiotensin-induced corticosteroid production. Gen Comp Endocrinol 54:333–338. Google Scholar
- M. Feuilloley, O. Lesouhaitier, C. Delarue, S. De Marchis, J. M. Conlon, H. A. Bern, and H. Vaudry . 1994. In vitro study of the effect of urotensin II on corticosteroid secretion in the frog Rana ridibunda. J Steroid Biochem Mol Biol 48:287–292. Google Scholar
- J. L. Fries, W. A. Murphy, J. Sueiras-Diaz, and D. H. Coy . 1982. Somatostatin antagonist analog increases GH, insulin, and glucagon release. Peptides 3:811–814. Google Scholar
- A. Ganguly and J. S. Davis . 1994. Role of calcium and other mediators in aldosterone secretion from the adrenal glomerulosa cells. Pharmacol Rev 46:417–447. Google Scholar
- W. Hanke and W. Kloas . 1994. Hormonal regulation of osmomineral content in Amphibia. Zool Sci 11:5–14. Google Scholar
- W. N. Holmes and J. Cronshaw . 1993. Some actions of angiotensin II in gallinaceous and anseriform birds. In “Avian Endocrinology”. Ed by P. J. Sharp , editor. Journal of Endocrinology Ltd. Bristol. pp. 201–216. Google Scholar
- S. Kapas, A. Purbrick, and J. P. Hinson . 1995. Role of tyrosine kinase and protein kinase C in the steroidogenic actions of angiotensin II, α-melanocyte-stimulating hormone and corticotropin in the rat adrenal cortex. Biochem J 305:433–438. Google Scholar
- J. F. Kocsis, M. H. Boyette, P. J. Mcllroy, and R. V. Carsia . 1994a. Regulation of aldosteronogenesis in domestic turkey (Meleagris gallopavo) adrenal steroidogenic cells. Gen Comp Endocrinol 96:108–121. Google Scholar
- J. F. Kocsis, P. J. Mcllroy, A. T. Chiu, R. J. Schimmel, and R. V. Carsia . 1994b. Properties of angiotensin II receptors of domestic turkey (Meleagris gallopavo) adrenal steroidogenic cells. Gen Comp Endocrinol 96:92–107. Google Scholar
- J. F. Kocsis, R. J. Schimmel, P. J. Mcllroy, and R. V. Carsia . 1995. Dissociation of increases in intracellular calcium and aldosterone production induced by angiotensin II (AII): evidence for regulation by distinct All receptor subtypes or isomorphs. Endocrinology 136:1626–1634. Google Scholar
- L. K. Malendowicz, G. G. Nussdorfer, A. Markowska, K. W. Nowak, and L. Torlinski . 1993. Effects of neuromedin-N on the pituitary-adrenocortical axis of dexamethasone-suppressed rats. Neuropeptides 24:1–4. Google Scholar
- I. Moeller, S. J. Bunn, and P. D. Marley . 1989. Actions of somatostatin on perfused bovine adrenal glands and cultured bovine adrenal medullary cells. Brain Res 484:192–202. Google Scholar
- G. Neri, L. K. Malendowicz, P. G. Andreis, and G. G. Nussdorfer . 1993. Thyrotropin-releasing hormone inhibits glucocorticoid secretion of rat adrenal cortex: in vivo and in vitro studies. Endocrinology 133:511–514. Google Scholar
- G. G. Nussdorfer 1996. Paracrine control of adrenal cortical function by medullary chromaffin cells. Pharmacol Rev 48:395–430. Google Scholar
- T. Reisine 1985. Multiple mechanisms of somatostatin inhibition of adrenocorticotropin release from mouse anterior pituitary tumor cells. Endocrinology 116:2259–2266. Google Scholar
- U. I. Richardson and A. Schonbrunn . 1981. Inhibition of adrenocorticotropin secretion by somatostatin in pituitary cells in culture. Endocrinology 108:281–290. Google Scholar
- J. Rosenberg, M. Pines, and S. Hurwitz . 1988. Regulation of aldosterone secretion by avian adrenocortical cells. J Endocrinol 118:447–453. Google Scholar
- K. S. Szalay 1981. Effect of pituitary intermediate lobe extract on steroid production by the isolated zona glomerulosa and fasciculata cells. Acta Physiol Hung 57:225–231. Google Scholar