Egg volume of a tropical sea urchin Echinometra mathaei is about one half that of other well-known species. We asked whether such a small size of eggs affected the timings of early developmental events or not. Cleavages became asynchronous from the 7th cleavage onward, and embryos hatched out before completion of the 9th cleavage. These timings were one cell cycle earlier than those in well-known sea urchins, raising the possibility that much earlier events, such as the increase in adhesiveness of blastomeres or the specification of dorso-ventral axis (DV-axis), would also occur earlier by one cell cycle. By examining the pseudopodia formation in dissociated blastomeres, it was elucidated that blastomeres in meso- and macromere lineages became adhesive after the 4th and 5th cleavages, respectively. From cell trace experiments, it was found that the first or second cleavage plane was preferentially employed as the median plane of embryo; the DV-axis was specified mainly at the 16-cell stage. Timings of these events were also one cell cycle earlier than those in Hemicentrotus pulcherrimus. The obtained results suggest that most of the early developmental events in sea urchin embryos do not depend on cleavage cycles, but on other factors, such as the nucleo-cytoplasmic ratio.
Early developmental events, such as cleavages or gastrulation, occur on a definite time schedule, provided that embryos are reared at a constant temperature. To explain such regularity in the timing of early events, existence of ‘developmental timers’ has been sometimes argued (Dan and Ikeda, 1971; Soll, 1979, 1983; Satoh and Ikegami, 1981b; Newport and Kirschner, 1982a; Satoh, 1985). Three factors involved in the proposed mechanisms seem to be important and to operate in a variety of developing systems. The first is the internal cytoplasmic oscillator, which is thought to begin to operate soon after fertilization. Timing of early cleavages is thought to be under the control of such a cytoplasmic oscillator (Dan and Ikeda, 1971). The second is the number of cleavage cycles or DNA replications. As shown in ascidian embryos, the process necessary for muscle cell differentiation is triggered after the occurrence of the 8th cleavage (Satoh and Ikegami, 1981a). The third is the ‘nucleo-cytoplasmic ratio’. The ratio doubles at each cleavage because the volume of blastomeres is reduced to one half, while the amount of nuclear material remains constant. In Xenopus embryos, for example, divisions of blastomeres become abruptly asynchronous (mid-blastula transition, MBT) after 12 cycles of synchronous cleavages. The timing of MBT is directed with such nucleo-cytoplasmic ratio (Newport and Kirschner, 1982a). Concomitantly with this transition, de novo synthesis of RNAs starts using the zygote genome, while maternal messages are predominantly used to promote development before MBT (Newport and Kirschner, 1982b).
However, the precise mechanism of ‘developmental timers’ remains unclear. This is mainly due to the small quantity of available data concerning the problem. It is still necessary to know which factor is crucial in individual events in a variety of developing systems. The sea urchin embryo is a preferable material for further analysis of the timing mechanism, since many stage-specific events have been known. For example, micromeres are formed at the 4th cleavage and divide unequally at the 5th cleavage. In Hemicentrotus pulcherrimus, dorso-ventral axis (DV-axis) is specified at some point from the 5th to 6th cleavage (Kominami, 1988). Ciliogenesis starts after the 8th cleavage (Masuda and Sato, 1984). Mespilia globulus embryos, as well as other well-known sea urchin species, hatch out from the fertilization envelope after completion of the 10th cleavage (Endo, 1966). Thus, the timing of many developmental events can be described in relation to the cleavage cycle.
Then, which factor is crucial in directing the timing of each event in sea urchin embryos? One of the ways to address this question is to reduce the volume of unfertilized or fertilized eggs. Interestingly, the timing of MBT in Xenopus embryos can be altered; if the egg cytoplasm is reduced to one half or one quarter of the initial volume, MBT is accelerated by one or two cell cycles, respectively (Newport and Kirschner, 1982a). This fact strongly suggests that the timing of MBT does not depend on the cleavage cycles, but on the ‘nucleo-cytoplasmic ratio’. Unfortunately, sea urchin eggs are too small to be manipulated as done in Xenopus or in ascidians (Yamada and Nishida, 1999). This is one of the reasons why few studies concerning timing mechanism have been reported in sea urchin embryos, in spite of its significance. To overcome this problem, we tried to examine the early development of a tropical sea urchin Echinometra mathaei whose eggs are fairly small (75–80 μm in diameter); the egg volume is about one half that of H. pulcherrimus (about 95 μm in diameter). The eggs of E. mathaei, we supposed might behave just like as the H. pulcherrimus eggs whose cytoplasm is reduced to one half.
MATERIALS AND METHODS
Adults of the sea urchin Echinometra mathaei were collected at the south district of Ehime prefecture during the breeding season. Animals were kept in aquaria with circulating seawater at 20°C. Milli-pore (pore size, 0.45 μm) filtered seawater (MFSW) was supplemented with 100 units/ml Penicillin G (Meiji Seika, Tokyo) and 50 μg/ml Streptomycin sulfate (Meiji Seika). Gametes were obtained by intracoelomic injection of 0.5 M KCl. Shed oocytes were rinsed twice with MFSW and inseminated with a dilute suspension of sperm. Embryos were reared in MFSW at 24°C.
Deprivation of fertilization envelope and dissociation of embryos
Unfertilized eggs were inseminated in MFSW containing 1 mM aminotriazole (Wako Pure Chemicals, Osaka) after the method reported by Showman and Foerder (1979). About 10 min after insemination, fertilized eggs were passed through nylon mesh (pore size, 62 μm) to deprive the fertilization envelope. At appropriate stages, embryos were collected with a hand-centrifuge, and transferred into 1 M glycine (isotonic to seawater). After 10 min, embryos were dissociated into single blastomeres in a small amount of the fresh medium with several strokes of gentle pipetting. Dissociated blastomeres were mounted on a glass slide, and photographed to measure the diameters of them.
Embryos were collected in a conical tube with a hand-centrifuge and fixed with Carnoi's fixative. Before staining, embryos were rinsed twice with phosphate-buffered saline (PBS). DAPI (Sigma, MO) was dissolved in DMSO at 10 mg/ml as stock and diluted with PBS at 2 μg/ml before use. Embryos were incubated in the staining solution at room temperature for 30 min, and rinsed three times with PBS for 10 min each. The specimens were mounted on a glass slide using glycerol for clarification, and examined under an epifluorescence microscope (BX50-FLA, Olympus, Tokyo).
Count of cell number in whole embryos
Numbers of cells in whole embryos were obtained after the method of Takahashi and Okazaki (1979). Briefly, 1 g orcein (Merck, Germany) was added to 50 ml acetic acid and boiled for 30 min. After cooling, 50 ml distilled-water was added, and the solution was filtered to remove the unsolved dye. Collected embryos were fixed with Carnoi's fixative, and stained with the solution for several days. The stained embryos were mounted on a glass slide with a small drop of lactic acid (Wako Pure Chemicals), covered with a cover slip and squashed. Spread nuclei were photographed and counted on photographic prints.
Observation of primary mesenchyme cells
Embryos were collected in a conical tube with a hand-centrifuge and fixed sequentially with cold methanol and ethanol (−20°C) for 20 min each. After being rinsed twice with PBS, embryos were stained with IG9, a monoclonal antibody that specifically binds to the surface of PMCs (Kominami and Takaichi, 1998), at room temperature for 1 hr. After the primary reaction, the embryos were rinsed three times with PBS for 10 min each, and then reacted with FITC-conjugated goat anti-mouse IgG (Fab fragment) antibody (Sigma) at room temperature for 1 hr. After three times rinse with PBS, the specimens were mounted on a glass slide with a drop of glycerol and examined under an epifluorescence microscope.
Observation of pseudopodia formation
To observe the lobopodium-like pseudopodia in dissociated blastomeres, embryos deprived of fertilization envelope were incubated in Ca2+, Mg2+-free artificial seawater for 10 min. Then, embryos were transferred into MFSW, and incubated for 1 min (Masui and Kominami, 2001). After these treatments, embryos were dissociated into single blastomeres with several strokes of gentle pipetting. Dissociated blastomeres were transferred into a plastic dish (55 mm in diameter, non-coated, Becton Dickinson Labware, NJ), and examined every cleavage stage (from the 2nd to 6th cleavage).
Injection of Lucifer Yellow CH
Embryos deprived of fertilization envelope were allowed to develop up to the 2-cell stage. Lucifer Yellow CH (Sigma) was iontophoretically injected into one of blastomeres as described (Kominami, 1988). The dye-injected embryos were reared in the dark up to the late gastrula or early prism stage (24–28 hr postfertilization). A small drop of 10% formalin was added into the culture dish to stop the ciliary movement of the embryos. The embryos were recovered from the culture dish, and examined under an epifluorescence microscope.
Early development of Echinometra mathaei embryo
Fig. 1 illustrates the early development of E. mathaei embryos. Comparing the egg of well-known sea urchin species (around 100 μm in diameter), the E. mathaei egg is rather small (Fig. 1A). The averaged diameters and standard deviations of eggs in three batches were 75.1±1.6 (n=30), 76.2±1.3 (n=30) and 78.0±2.1 (n=30). Fertilization envelope was not highly elevated. Due to the narrow perivitelline space, blastomeres could not resume the spherical shape after cleavages (Fig. 1B). At the 4th cleavage, micromeres were formed at the vegetal pole (Fig. 1C, arrow). From the 5th cleavage onward, a narrow blastocoel was noticed. Embryos began rotation at about 8 hr, and hatched out from the fertilization envelope around 9 hr post-fertilization (Fig. 1D). At 16–17 hr, ingressed PMCs became noticeable owing to expansion of the blastocoel (Fig. 1F). At about 18 hr, embryos started gastrulation (Fig. 1G). At 24 hr, the archenteron tip reached the apical plate (Fig. 1H). By this stage, larval skeletons had already formed, and dorsoventral axis of the embryo was clearly seen (Fig 1I). At 36 and 48 hr postfertilization, embryos developed into the early and four-armed plutei, respectively (Fig. 1J, K).
Time schedule of cleavages
Fig. 2 shows the time schedule of cleavages. Until the 5th cleavage, timing of each cleavage could be determined in intact embryos. The 1st cleavage occurred at 70–80 min postfertilization, then the 2nd to 5th cleavages followed with intervals of 40–45 min (Fig. 2, arrowheads). It is of note that formation of small micromeres was considerably delayed. Thus, the embryos were composed of 28 cells around the 4th hr of development. From the 6th cleavage onward, the timing of cleavage could not be determined in intact embryos, due to the tight adhesion of blastomeres and opaqueness of embryos. Therefore, the durations of later cleavages (Fig. 2, both-sided arrows) were estimated from the diameters of dissociated blastomeres as described below.
First, the diameters of ancestor blastomeres of each lineage, i.e., meso-, macro- and micromeres, were measured at the 16-cell stage. The averaged diameters and standard deviations of meso-, macro- and micromeres were 28.4±1.3 (n=15), 33.8±3.9 (n=10) and 21.2±3.6 μm (n=10), respectively. Next, embryos were dissociated into single blastomeres every hour from 5 to 8 hr postfertilization, and the diameter of dissociated blastomeres were measured (Fig. 3). From the diameters of ancestor blastomeres, the diameter of blastomeres during later cleavage stages can be calculated. For example, the diameter of blastomeres in macromere lineage after the 6th cleavage is obtained by dividing 33.8 with 1.262. On the histograms of blastomere diameters, the calculated diameters in each lineage are indicated with arrows accompanying the number of each cleavage cycle.
At 5 hr postfertilization, most of blastomeres completed the 6th cleavage, while some did not divide yet (Fig. 3A). Small micromeres were first observed at this time (indicated by ‘s-mic’ in Fig. 3A). At 6 hr postfertilization, a portion of blastomeres had already undertaken the 7th cleavage (Fig. 3B). By the 7th hr of development, embryos had completed the 7th cleavage (Fig. 3C). At the 8th hr of development, most of blastomeres seem to have undertaken the 8th cleavage (Fig. 3D). Taking these data into consideration, presumed periods of the 6th, 7th and 8th cleavages were estimated as shown in Fig. 2 (both-sided arrows). From diameters of the dissociated blastomeres, it was also elucidated that embryos had not completed the 10th cleavage even at the late gastrula stage (24 hr, Fig. 3E).
Initiation of asynchronous cleavage
In H. pulcherrimus embryos, division of blastomeres becomes asynchronous from the 8th cleavage onward (Masuda and Sato, 1984). In contrast to this, the data shown in Fig. 2 and 3 suggest that the division becomes asynchronous from the 6th or 7th cleavage onward in E. mathaei embryos. However, the possibility remains that the broad distribution of blastomere diameters merely reflects the asynchrony among embryos.
To ascertain the timing of the initiation of asynchronous cleavage, individual embryos were observed by staining the nuclei with DAPI (Fig. 4). The 4th cleavage was almost synchronous. In some embryos, divisions of the animal blastomeres occurred slightly earlier than those of the vegetal blastomeres (Fig. 4A-A″); the difference was 5 min at most. Synchrony in the 5th cleavage seemed to be lost to some extent (Fig. 4B-B″), but the difference in the occurrence of cleavages was still within 5–10 min. In the embryo shown in Fig. 4C-C″, most of the nuclei in the animal hemisphere are in prophase or metaphase of the 6th cleavage, while the nuclei in the vegetal hemisphere still remain in prophase. Thus, synchrony of cleavages in each hemisphere seemed to be retained up to the 6th cleavage, although difference in the timing of cleavages was noticed between the animal and vegetal hemispheres. However, the occurrence of the 7th cleavages was asynchronous among the blastomeres even in the same lineage, as shown in Fig. 4D-D″. Nuclei of some blastomeres are in metaphase or anaphase, while the others are in S-phase even in the same hemisphere. Together with the distribution of blastomere diameters shown in Fig. 3, these data indicate that cleavages become asynchronous from the 7th cleavage onward in E. mathaei embryos, one cycle earlier than in H. pulcherrimus.
Increase in cell number after hatching
E. mathaei embryos hatched out from the fertilization membrane around the 9th hr of development. After hatching, nuclei of embryonic cells could be clearly stained with aceto-orcein, probably due to the tight packaging of chromosomes (Rowland and Rill, 1987). Fig. 5 shows change in the number of cells per embryo from 9 to 48 hr. From this figure, it is clear that the 9th cleavage had already started at 9 hr postfertilization. However, the completion of the 9th cleavage was around 15 hr. The 10th cleavage still continued even in the late gastrulae (24 hr). This coincides well with the results obtained from the measurement of the diameters of dissociated blastomeres (Fig. 3E). In the pluteus-stage embryo (48 hr), about two thirds of constituent cells had undertaken the 11th cleavage.
In H. pulcherrimus, embryos hatch out after the 10th cleavage and develop into the late gastrula around the completion of the 11th cleavage (Kominami, 2000). Therefore, the timings of hatching out and the completion of gastrulation in E. mathaei embryos are also earlier by one cell cycle than those in H. pulcherrimus.
Number of primary mesenchyme cells
As described above, the asynchronous cleavage, hatching out and gastrulation in E. mathaei started one cell cycle earlier than those in well-known sea urchins. As a result, the late-gastrula stage embryo of E. mathaei was composed of only several hundreds of cells. These seem to be ascribed to the small size of E. mathaei eggs. As is well known, division schedule of blastomeres in micromere lineage is independent of meso- and macromere lineages. Therefore, it is interesting to know whether the size of eggs affect the division and differentiation schedule of blastomeres in micromere lineage.
Relative size of micromeres formed at the 16-cell stage seemed to be somewhat larger than those in other sea urchins (Fig. 1C). Further, the size of four micromeres in an embryo was fairly variable in a considerable portion of embryos (Fig. 6A). As shown in Fig. 6B, B′ (at the early gastrula stage), PMCs are rather large in diameter, compared with other embryonic cells. At the pluteus stage, a well-developed larval skeleton was formed (Fig. 6C-C″). Although the contour of PMCs was scarcely noticed, a small number of PMCs could be demarcated even at the pluteus stage (arrows in C′ and C″). The diameters of such PMCs did not differ from those observed at the early gastrula stage.
As shown in Fig. 7, the number of PMCs obtained at the early gastrula stage was fairly variable among embryos. The maximum number was 46. The embryo containing only 19 PMCs was also observed. The average was 31.1 with a standard deviation of 5.1. It is of note that the embryos having 32 PMCs were frequently observed.
Pseudopodia formation during early cleavage stage
The results described above raised the possibility that much earlier events would also occur earlier by one cell cycle. As reported, sea urchin blastomeres form lobopodium-like pseudopodia when embryos are dissociated into single blastomeres in the presence of Ca2+ (Masui and Kominami, 2001). In H. pulcherrimus, the timings of such pseudopodia formation in meso-, macro- and micromere lineage are 5th, 6th and 4th cleavage, respectively.
Up to the 8-cell stage, dissociated blastomeres of E. mathaei embryos remained spherical and did not form pseudopodia at all (Figs. 8A; 4-cell stage and B; 8-cell stage). After the occurrence of the 4th cleavage, a considerable portion of mesomeres formed pseudopodia as well as micromeres (Fig. 8C, D). The pseudopodia are not mere the cytoplasmic blebs but show adhesive properties (Fig. 8D, arrowheads). The mesomere shown in Fig. 8D is elon-gated due to the adhesion to the substratum. After the occurrence of the 5th cleavage, most of blastomeres, including those in macromere lineage, formed pseudopodia (Fig. 8E, F). Almost all the blastomeres of 60-cell stage embryos showed extensive pseudopodia formation and became irregular in shape after dissociation (Fig. 8G, H). This observation shows that blastomeres of E. mathaei embryos in meso- and macromere lineages form pseudopodia after the occurrence of 4th and 5th cleavage, respectively. These timings in E. mathaei are earlier by one cell cycle than those in H. pulcherrimus.
Specification of dorso-ventral axis
When one of blastomeres of the 2-cell stage embryo was labeled in H. pulcherrimus, eight labeling patterns were observed at the prism stage with respect to the DV-axis (Kominami, 1988). This observation suggested that the DV-axis (oral-aboral axis) would be specified at some point from the 5th to 6th cleavage. It is of interest that this timing coincides well with the timings of the initiation of pseudopodia formation. As described above, some blastomeres of E. mathaei embryos begin to form pseudopodia, i.e., become adhesive from the 4th cleavage onward. Therefore, it is naturally supposed that the DV-axis is specified around the 16-cell stage and that only four labeling patterns (dorsal, ventral, left and right) would appear, if one of blastomeres is labeled at the 2-cell stage.
Fig. 9 shows examples of the labeled embryos observed at the late gastrula or early prism stage. In the embryo shown in Fig. 9A, A′, the right half of the embryo was labeled with the fluorescent dye; the first cleavage plane coincides with the median plane of the embryo. In contrast to this, the first cleavage plane is perpendicular to the median plane in the embryo shown in Fig. 9B, B′. Likewise in H. pulcherrimus, ‘oblique’ labeling patterns were also observed. The embryo shown in Fig. 9C, C′, most of the dorsal part and small ventral part (left side) were labeled (named as dorso-lateral (L) labeling). On the other hand, most of the ventral part and small dorsal part (left side) were labeled in the embryo shown in Fig. 9D, D′ (ventro-lateral (L) labeling). In these two embryos, one of the 5th cleavage planes of the mesomeres had been employed as the median plane of embryos.
Table 1 summarizes the number of embryos classified with respective labeling patterns. Of interest, the embryos with ‘coinciding’ or ‘perpendicular’ pattern were more frequently observed than the embryos with oblique labeling patterns in every series of experiments (I–III). The difference in the numbers (38:13) was statistically significant (Z=3.54, p<0.005). This indicates that the 1st or 2nd cleavage plane is predominantly employed as the median plane of embryos, and suggests that the DV-axis is specified at the 16-cell stage in a majority of embryos. Another significant difference is found in the frequencies of appearance between coinciding and perpendicular labeling patterns. The difference in those numbers (10:28) is also statistically significant (Z=2.76, p<0.01), indicating that the 2nd cleavage plane is preferentially employed as the median plane of embryos rather than the 1st cleavage plane. Difference in the numbers of complementary labeling patterns, e.g., left: right (6:4), or ventral: dorsal (18:10), was not statistically significant.
Number of embryos showing respective labeling patterns
Initiation of asynchronous cleavage
In these years, several reports have referred to the significance of the late blastula stage in sea urchin development. ‘Early mRNAs’, including those encoding tolloid and BMP-1, are transcribed during the late blastula stage (Reynolds et al., 1992). Presumptive endodermal cells require cell contacts for their differentiation, but become to be able to fulfill their fate without further cell contact (Chen and Wessel, 1996). Specification of mesodermal tissues, such as pigment cells or blastocoelar cells, occurs also during late cleavage stages (Kominami, 1998, 2000; McClay et al., 2000; Sweet et al., 2002). Thus, the basic body plan of future embryos seems to be established during late cleavage stages. However, many problems remain unsolved concerning the events occurring during late cleavage stages. Presently, much more information is necessary for the comprehensive understanding of sea urchin development.
One of the unsolved problems is the nature of MBT in sea urchin embryos. Unlike in Xenopus embryos, it has not been characterized what factors affect the transition from synchronous to asynchronous cleavage, probably due to the difficulty in manipulating unfertilized or fertilized eggs. In the present study, we used the difference in the egg volume among sea urchin species to overcome the difficulty. As revealed in the present study, cleavages become asynchronous from the 7th cleavage in E. mathaei embryos (Figs. 2, 3 and 4), one cell cycle earlier than in H. pulcherrimus (Masuda and Sato, 1984), while the egg volume of the former species is about one half to that of the latter. This suggests that the nucleo-cytoplasmic ratio is important in directing the timing of the transition as in Xenopus embryos.
Timing of hatching and gastrulation
It is generally accepted that sea urchin embryos hatch out from the fertilization envelope after the completion of the 10th cleavage (Endo, 1966). Surprisingly, E. mathaei embryos had not complete the 9th cleavage by the hatching stage, hence the embryos contained only 290 cells (Fig. 5). Even at the late gastrula stage, all the constituent cells have not undertaken 10 cycles of cell divisions (Fig. 5). These suggest that neither the number of cleavage cycles nor DNA replications directs the timing of hatching or progress of gastrulation.
From the present study, it cannot be distinguished which factor, a cytoplasmic oscillator or the nucleo-cytoplasmic ratio, is crucial in directing the timing of such events. As reported earlier (Matsumoto et al., 1988), the timings of hatching out and onset of gastrulation are directed with different timers; the timing of hatching is directed with the timer that begins to operate soon after fertilization and governs the cleavage cycles, while the timing of the onset of gastrulation seems to be directed with a timer that begins to operate after cleavages become asynchronous. Time schedule of early cleavages, i.e., occurrence of the first cleavage and cleavage intervals, does not differ between E. mathaei and H. pulcherrimus. Embryos of H. pulcherrimus hatch out around 12 hr and begin to gastrulate at about 16–17 hr post-fertilization. On the other hand, E. mathaei embryos hatch out around 9 hr and begin to gastrulate at about 18 hr post-fertilization (Fig. 1G). Thus, the relative time length from hatching to the initiation of gastrulation is quite different between these two species. This supports the idea that the timings of hatching and onset of gastrulation are directed with different timing mechanisms.
Number of cell divisions in micromere lineage
Some data are available on the numbers of PMCs in different sea urchin species. In Clypeaster japonicus, the number ranged from 55 to 75 (Takahashi and Okazaki, 1979). In Lytechinus variegatus, the number of PMCs was reported to be 60–64 (Ettensohn and McClay, 1988). In H. pulcherrimus, the averaged number were 50–60, although the number varied to some extent among batches of embryos (Kominami and Takaichi, 1998). Thus, the number of PMCs in well-known sea urchin species is around 60. These indicate that each micromere generally divides four times and give rise to nearly 16 PMCs before they form the larval skeleton. In contrast to this, each micromere divides only three times and produces 8 PMCs in E. mathaei embryos, since the total number of PMCs is around 32 (Fig. 7).
The size of differentiated PMCs was almost the same as far as examined on the antibody-stained specimens (Fig. 6B′). It is important to remember that E. mathaei embryos sometimes form extreme large micromeres as shown in Fig. 6A. Such micromeres might be able to undergo four rounds of cell divisions. If embryos form one or two larger micromeres, the number of PMCs will be around 40 or 48, respectively. In fact, such numbers of PMCs were observed in some embryos (Fig. 7). On the contrary, if two smaller micromeres are formed, the number will be around 24. The embryos containing such number of PMCs were also observed (Fig. 7). Therefore, differentiation of PMC does not strictly depend on the number of division cycles or DNA replications. In another words, the number of cell divisions depends on the volume of micromeres (Yamada and Nishida, 1999).
In addition to say, the monoclonal antibody used in this study was raised against H. pulcherrimus embryos (Kominami and Takaichi, 1998). Nonetheless, the antibody binds specifically to the surface of PMCs in E. mathaei. The antibody used in this study recognizes the molecules similar to msp130 (Leaf et al., 1987; Anstrom et al., 1987). Such molecules seem to prevail in a variety of sea urchins.
Timing of the initiation of close cell contact
As shown in the present study, blastomeres in mesoand macromere lineages began to form lobopodium-like pseudopodia after the 4th and 5th cleavage, respectively (Fig. 8). These timings are earlier by one cell cycle than in H. pulcherrimus. Since the egg volume of E. mathaei is about one half to that of H. pulcherrimus, it is naturally supposed that the timing of pseudopodia formation is directed with a nucleo-cytoplasmic ratio. More strictly speaking, the nucleus-cell volume ratio (not the nucleo-cytoplasmic ratio) would be crucial in directing the timing (Masui and Kominami, 2001). When blastomeres become adhesive, the ratio exceeded 0.1 irrespective of the blastomere lineage in H. pulcherrimus. This is also the case in starfish embryos, while the definite value was 0.06 (Masui et al., 2001). Unfortunately, we could not obtain enough data on the change in the nucleus-cell volume ratio, since it was difficult to identify the lineage of dissociated blastomeres in E. mathaei. To clarify the properties of the developmental timer that directs the initiation of close cell contact, it is necessary to obtain the quantitative data on the change in the nucleus-cell volume ratio also in E. mathaei.
Specification of dorso-ventral axis
It is still a debated problem when the DV-axis of sea urchin embryos is specified (Henry, 1998), since the axis is labile (Coffman and Davidson, 2001). Further, the relationship between the first cleavage plane and the median plane of embryos, which is an indicator that shows the timing of the DV-axis specification, varies among sea urchin species (Kominami, 1988; Cameron et al., 1989; Henry et al., 1990, Summers et al., 1996). In the direct developing sea urchin Heliocidaris erythrogramma, the DV-axis is specified prior to the 1st cleavage, and thought to be specified even in the unfertilized egg (Henry et al., 1990). In indirect developing sea urchins, however, many reports support the idea that the DV-axis is specified through cell-cell interactions during early cleavage stage. This is also the case in the starfish Asterina pectinifera (Kominami, 1983). It is of note that vegetal hemisphere play an important role in the specification of DV-axis during early cleavage stages. In Psammechinus miliaris, the animal half isolated at the 8-cell stage differentiates into a permanent blastula that does not show any sign of DV-axis. On the other hand, the animal halves isolated at the 32-cell stage develop into the blastulae having a depression that resembles the stomodaeum formed in normal embryos (Hörstadius, 1973).
Like in H. pulcherrimus embryos, oblique labeling patterns were observed also in E. mathaei embryos (Fig. 9, Table 1). However, the 1st or 2nd cleavage plane was preferentially employed as the median plane (Table 1). This suggests that the DV-axis is specified at the 4th cleavage rather than the 5th cleavage, one cell cycle earlier than in H. pulcherrimus, of which egg volume is twice that of E. mathaei. Further, the inclination was observed that the 2nd cleavage plane was more frequently employed as the median plane of embryos (Table 1). Probably, the immediately preceding cleavage plane is easy to be employed as the median plane of the embryo. These may also suggest that specification of the DV-axis needs close contact of blastomeres. It should be emphasized that the timing of DV-axis specification coincides well with the timing when blastomeres become adhesive (Fig. 8). In another words, the timings of the DV-axis specification and the initiation of close cell contact might be directed by the same factor, i.e., the nucleo-cytoplasmic ratio.
Thus, the obtained results suggest that the timings of early developmental events in sea urchin embryos are mostly under the control of nucleo-cytoplasmic ratio (or nucleus-cell volume ratio). In the present study, timing mechanisms was studied by means of comparison among sea urchin species, instead of manipulations. Although attention should be paid in interpreting the results obtained from such comparison, the method would help us to reveal new aspects of the unsolved problems that cannot be addressed with the ordinary methods.
We would express sincere thanks to Professor. Y. Yanagisawa, Ehime University, for providing us the facility in collecting animals. We also thank all the members of our laboratory for their collaboration.
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