Monthly samples of arthropod remains (n = 1,190 culled parts) collected over a year from under a Nycteris thebaica night roost in the Kenneth Stainbank Nature Reserve (South Africa) show that prey eaten by bats vary significantly by order but not by season. Nevertheless, there was a significant interaction between the prey category and season, suggesting that these two factors are not independent from each other. Coleoptera (49.6% in the culled parts, calculated as percent composition) dominated in spring (September—November), Orthoptera (38.8%) in summer (December—February), Hemiptera (42.8%) in autumn (March—May), and Lepidoptera (36.3%) in winter (June—August). The diet also included a frog and a small fish (n = 2 parts).