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1 December 2002 A New Species of Long-Eared Bat (Chiroptera, Vespertilionidae) from Sardinia (Italy)
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We describe a new species of long-eared bat, genus Plecotus, from the island of Sardinia (Italy). The new species is clearly distinguishable from other European Plecotus species by its mitochondrial 16S rRNA gene (4.1–9.6% sequence divergence) as well as by a unique combination of morphological characters such as brownish colour of dorsal pelage, a relatively large thumb and thumb claw, an almost cylindrical form of the penis and the characteristic shape of the baculum. The most important morphological diagnostic character is a relatively long (≥ 18 mm) and wide (≥ 6 mm) tragus. The new species is currently known from three localities on Sardinia. In addition to the new species we discovered a lineage of P. auritus, which is substantially differentiated from continental P. auritus at subspecific level (1.2–2.7% of sequence divergence of the 16S rRNA gene). The existence of these two endemic bat taxa on Sardinia highlights the island's importance in the conservation of the European bat community.



S. Anderson 1960. The baculum in Microtinae rodents. University of Kansas Publications of the Museum of Natural History, 12: 181–216. Google Scholar


T. E. M. Barratt , R. Deaville , T. M. Burland , M. W. Bruford , G. Jones , P. A. Racey , and R. K. Wayne . 1997. DNA answers the call of the pipistrelle bat species. Nature, 387: 138–139. Google Scholar


R. D. Bradley , and R. J. Baker . 2001. A test of the genetic species concept: cytochrome-b sequences and mammals. Journal of Mammalogy, 82: 960–973. Google Scholar


V. Castella, M. Ruedi, L. Excoffier, C. Ibáñez, R. Arlettaz, and J. Hausser . 2000. Is the Gibraltar Strait a barrier to gene flow for the bat Myotis myotis (Chiroptera: Vespertilionidae)? Molecular Ecology, 9: 1761–1772. Google Scholar


S. J. B. Cooper , P. R. Day , T. B. Reardon , and M. Schulz . 2001. Assessment of species boundaries in Australian Myotis (Chiroptera: Vespertilionidae) using mitochondrial DNA. Journal of Mammalogy, 82: 328–338. Google Scholar


O. De Paz 1994. Systematic position of Plecotus (Geoffroy, 1818) from the Iberian Peninsula (Mammalia: Chiroptera). Mammalia, 58: 423–432. Google Scholar


B. Đulić 1980. Morphological characteristics and distribution of Plecotus auritus and Plecotus austriacus in some regions of Yugoslavia. Pp. 151–161, in Proceedings of the 5th International Bat Research Conference ( D. E. Wilson and A. L. Gardner , eds.). Texas Tech Press, Lubbock, 434 pp. Google Scholar


J. Felsenstein 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39:783–791. Google Scholar


U. Häussler , and M. Braun . 1991. Sammlung einheimischer Fledermäuse (Mammalia: Chiroptera) des Staatlichen Museums für Naturkunde Karlsruhe. Teil 2: Plecotus. Carolinea, 49: 101–114. Google Scholar


S. B. Hedges 1992. The number of replications needed for accurate estimation of the bootstrap P value in phylogenetic studies. Molecular Biology and Evolution, 9: 66–369. Google Scholar


C. Ibáñez , and R. Fernandez . 1985. Systematic status of the long-eared bat Plecotus teneriffae Barrett-Hamilton, 1907 (Chiroptera; Vespertilionidae). Säugetierkundliche Mitteilungen 32: 143–149. Google Scholar


G. Jones, and S. M. Parus . 1993. Bimodal echolocation in pipistrelle bats: are cryptic species present? Proceedings of the Royal Society of London, B 251:119–125 Google Scholar


A. Kiefer , and M. Veith . 2002. A new species of long-eared bat from Europe (Chiroptera: Vespertilionidae). Myotis, 39: 5–16 [for 2001]. Google Scholar


A. Kiefer , J. Kosuch , M. Veith , F. Mayer , and O. Von Helversen . 2002. Conflicting molecular phylogenies of European long-eared bats (Plecotus) can be explained by cryptic diversity. Molecular Phylogenetics and Evolution, 25: 557–566. Google Scholar


D. Kock 2002. The publication dates of Plecotus alpinus Kiefer and Veith, 2002 and of Plecotus microdontus Spitzenberger 2002. Acta Chiropterologica, 4: 219–220. Google Scholar


S. V. Kruskop , and L. A. Lavrenchenko . 2000. A new species of long-eared bat (Vespertilionidae, Chiroptera) from Ethiopia. Myotis, 38: 5–17. Google Scholar


B. Lanza 1959. Chiroptera. Pp. 187–473, in Fauna d'ltalia, Vol. IV, Mammalia ( A. Toschi and B. Lanza , eds.). Edizioni Calderini, Bologna, viii + 488 pp. Google Scholar


B. Lanza 1960. Su due specie criptiche di Orecchione: ‘Plecotus auritus’ (L.) e ‘P. wardi’ Thomas (Mamm.; Chiroptera). Monitore Zoologico Italiano, LXVIII, 1–2: 7–23. Google Scholar


P. J. Lockhart , M. A. Steel , M. D. Hendy , and D. Penny . 1994. Recovering evolutionary trees under a more realistic model of sequence evolution. Molecular Biology and Evolution, 11: 605–612. Google Scholar


E Mayer , and O. Von Helversen . 2001. Cryptic diversity in European bats. Proceedings of the Royal Society London, B 268: 1825–1832. Google Scholar


A. J. Mitchell-Jones , G. Amori , W. Bogdanowicz , B. Kryštufek , P. J. H. Reijnders , F. Spitzenberger , M. Stubbe , J. B. M. Thissen , V. Vohralík , and J. Zima . 1999. The atlas of European mammals. Academic Press, London, xi + 484 pp. Google Scholar


M. Mucedda, M. L. Bertelli, and E. Pidinchedda . 1999. Risultati di 6 anni di censimento dei pipistrelli in Sardegna. Atti del 1° Convegno Italiano sui Chirotteri, Castellazzara: 105–114. Google Scholar


M. Mucedda , E. Pindinchedda , A. Kiefer , and M. L. Bertelli . 2002. Prima segnalazione in Sardegna (Italia) dell'Orecchione meridionale Plecotus austriacus (Chiroptera, Vespertilionidae) e conforma della presenza nell'isola dell' Orecchione comune Plecotus auritus. Memorie del Museo Riserva Naturale Orientata di Onferno, 2: 5–8. Google Scholar


M. Nikaido , K. Kawai , Y. Cao , M. Harada , S. Tomita , N. Okada , and M. Hasegawa . 2001. Maximum likelihood analysis of the complete mitochondrial genomes of eutherians and a reevaluation of the phylogeny of bats and insectivores. Journal of Molecular Evolution, 53: 508–516. Google Scholar


S. Palumbi , A. Martin , S. Romano , W. O. Mc-Millan , L. Stice , and G. Grabowski . 1991. The simple fool's guide to PCR. Version 2. Department of Zoology and Kewalo Marine Laboratory, University if Hawaii, Honolulu, 44 pp. Google Scholar


D. Posada , and K. A. Crandall . 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics, 14: 817–818. Google Scholar


M. B. Qumsiyeh 1985. The bats of Egypt. Special Publications the Museum Texas Tech University, 23: 1–102. Google Scholar


N. Saitou , and M. Nei . 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4: 406–425. Google Scholar


W. Schober , and E. Grimmberger . 1989. A guide to bats of Britain and Europe. Hamlyn Publishing Group Limited, London, 224 pp. Google Scholar


M. E Smith , and J. L. Patton . 1993. The diversification of South American murid rodents: evidence from mitochondrial DNA sequence data for the akodontine tribe. Biological Journal of the Linnaean Society, 50: 149–177. Google Scholar


F. Spitzenberger , E. Haring , and N. Tvrtkovic . 2002. Plecotus microdontus (Mammalia, Vespertilionidae), a new bat species from Austria. Natura Croatica, 11: 1–18. Google Scholar


F. Spitzenberger , J. Pialek , and E. Haring . 2002. Systematics of the genus Plecotus (Mammalia, Vespertilionidae) in Austria based on morphometric and molecular investigations. Folia Zoologica, 50: 161–172. Google Scholar


R. E. Stebbings 1967. Identification and distribution of bats of the genus Plecotus in England. Journal of Zoology (London), 153: 291–310. Google Scholar


P. P. Strelkov 1988. Brown (Plecotus auritus) and grey (P. austriacus) bats (Chiroptera, Vespertilionidae) in the USSR. — Communication I. Zoologicheskiï Zhurnal, 67: 90–101. [in Russian]. Google Scholar


P. P. Strelkov 1989a. Brown (Plecotus auritus) and grey (P. austriacus) bats (Chiroptera, Vespertilionidae) in the USSR. — Communication II. Zoologicheskiï Zhurnal 67: 287–292. [in Russian]. Google Scholar


P. P. Strelkov 1989b. New data on the structure of baculum in Palaearctic Bats. Pp. 87–94, in Proceedings of the 4th European Bat Research Symposium ( V. Hanák, I. Horáček, and J. Gaisler , eds.). Charles University Press, Praha, 718 + xxii pp. Google Scholar


D. L. Swofford 2001. PAUP*: phylogenetic analysis using parsimony (and other methods), version 4.08b. Sinauer Associates, Sunderland/Massachusetts. Google Scholar


S. M. Swift 1998. Long-eared bats. T. & A. D. Poyser Ltd., London, 182 pp. Google Scholar


K. Tamura , and M. Nei . 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzee. Molecular Biology and Evolution, 10: 512–526. Google Scholar


J. D. Thompson , T. J. Gibson , F. Plewniak , F. Jeanmougin , and D. G. Higgins . 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 24: 4876–1882. Google Scholar


G. Topál 1958. Morphological studies on the os penis of bats in the Carpathian Basin. Annales Historico-Naturales Musei Nationalis Hungarica, 50: 331–342. Google Scholar


O. Von Helversen 1989. Bestimmungsschlüssel für die europäischen Fledermäuse nach äuβeren Merkmalen. Myotis, 27: 41–60. Google Scholar


O. Von Helversen , K.-G. Heller , F. Mayer , A. Nemeth , M. Volleth , and P. Gompkötö . 2001. Crytic mammalian species: a new species of whiskered bat (Myotis alcathoe n. sp.) in Europe. Naturwissenschaften, 88: 217–223. Google Scholar


R. Weid , and O. Von Helversen . 1987. Ortungsrufe europäischer Fledermäuse beim Jagdflug im Freiland. Myotis, 25:5–27. Google Scholar


J. Worthington Wilmer , and E. M. Barratt . 1996. A non-lethal method of tissue sampling for genetic studies of chiropterans. Bat Research News, 37: 1–3. Google Scholar


M. Yoshiyuki 1991. A new species of Plecotus (Chiroptera, Vespertilionidae) from Taiwan. Bulletin National Science Museum Tokyo, A 17: 189–195. Google Scholar
© Museum and Institute of Zoology PAS
Mauro Mucedda, Andreas Kiefer, Ermanno Pidinchedda, and Michael Veith "A New Species of Long-Eared Bat (Chiroptera, Vespertilionidae) from Sardinia (Italy)," Acta Chiropterologica 4(2), 121-135, (1 December 2002).
Received: 8 November 2002; Accepted: 1 November 2002; Published: 1 December 2002

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